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应用
Sigma的壳聚糖酶已用于几丁质和壳聚糖的偏振光刺激的酶促水解。
生化/生理作用
壳聚糖酶催化壳聚糖中D-氨基葡萄糖(GlcN-GlcN)残基之间的β-1,4-键的内水解。据报道,来自链霉菌属的酶还能水解部分乙酰化壳聚糖中的GlcNAc-GlcN键。
单位定义
一个酶活性单位是指在37 °C、pH 5.5下,每分钟从壳聚糖中释放1.0 μmole还原糖(以D-葡萄糖胺当量计)所需的酶量。
外形
溶于含50 mM乙酸钠的50%甘油溶液中
制备说明
溶于10 mL的0.1 M乙酸缓冲液,pH值为5.5
其他说明
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
FEMS Microbiology Letters, 65, 31-31 (1989)
Carbohydrate research, 343(18), 3117-3119 (2008-10-01)
Illumination with white linearly polarized light (WLPL) stimulated chitinase and chitosanase in their degradation of chitin and chitosan, respectively. Enzymes were illuminated at room temperature in separate vessels, then admixed in reactors containing polysaccharides. Hydrolysis of chitosan to glucosamine followed
A new reaction for colorimetric determination of carbohydrates.
Analytical biochemistry, 47(1), 273-279 (1972-05-01)
The Biochemical journal, 311 ( Pt 2), 377-383 (1995-10-15)
Chitosanase was produced by the strain of Streptomyces lividans TK24 bearing the csn gene from Streptomyces sp. N174, and purified by S-Sepharose and Bio-Gel A column chromatography. Partially (25-35%) N-acetylated chitosan was digested by the purified chitosanase, and structures of
Frontiers in nutrition, 8, 756218-756218 (2021-11-02)
Canthaxanthin is a reddish-orange xanthophyll with strong antioxidant activity and higher bioavailability than carotenes, primarily used in food, cosmetics, aquaculture, and pharmaceutical industries. The spiking market for natural canthaxanthin promoted researchers toward genetic engineering of heterologous hosts for canthaxanthin production.
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