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无菌性
sterile-filtered
质量水平
表单
liquid
技术
cell culture | mammalian: suitable
cryopreservation: suitable
杂质
endotoxin, tested
运输
dry ice
储存温度
−20°C
一般描述
细胞冷冻培养基是防冻剂DMSO与胎牛血清的组合。但无血清培养基可用甲基纤维素和泊洛沙姆188以及其他添加剂（如抗坏血酸、α-生育酚和脯氨酸）替代血清。
应用
DMSO无血清1x细胞冷冻培养基已用于冷冻人类神经祖细胞(hNPCs)，嗅鞘细胞(OECs)和视网膜色素上皮细胞(RPE)。
其他说明
含 MEM、二甲基亚砜 (8.7%) 和甲基纤维素的完全即用型培养基。根据 Waymouth 的配方配制。
相关产品
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
Kai Pollard et al.
Scientific data, 7(1), 184-184 (2020-06-21)
Nerve sheath tumors occur as a heterogeneous group of neoplasms in patients with neurofibromatosis type 1 (NF1). The malignant form represents the most common cause of death in people with NF1, and even when benign, these tumors can result in
Cryopreservation of cells using defined serum-free cryoprotective agents
Volbers JC, et al.
Current Directions in Biomedical Engineering, 2(1), 315-318 (2016)
Simple freezing procedure for storage in serum-free media of cultured and tumor cells of mouse.
Waymouth, C. and Varnum, D.
TCA Manual, 2(1), 311-313 (1976)
商品
用于细胞系冷冻保存的良好细胞库操作实践概述。
Overview of good cell banking practices for cell line cryopreservation purposes.
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