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形式
powder
IVD
for in vitro diagnostic use
技术
cell culture | mammalian: suitable
储存温度
2-8°C
一般描述
CMRL-1066 is a chemically defined medium developed in the late 1950′s by Connaught Medical Research Laboratories. A less complex and extensively modified version of Medium 199, CMRL-1066 was designed initially for use with L-strain cells in unsupplemented culture. It has been used to maintain monolayer growth of permanent cell lines for years without protein supplementation. Although developed for use in serum free cell culture, CMRL-1066 can be supplemented with serum and used to support the growth of many types of cells.
应用
CMRL-1066 has been used for culture of blastomeres and control embryos in vitro.
公式变量
With L-glutamine. Without sodium bicarbonate.
数量
Formulated to contain 9.8 grams of powder per liter of medium.
重悬
Supplement with 2.2 g/L sodium bicarbonate.
Cellular characterization of blastocysts derived from rabbit 4-, 8-and 16-cell embryos and isolated blastomeres cultured in vitro.
Tao T and Niemann H.
Human Reproduction, 15(4), 881-889 (2000)
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