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产品名称
StableCell™ DMEM - 高葡萄糖, With 4500 mg/L glucose, stable glutamine, and sodium bicarbonate, without sodium pyruvate., liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
适用性
suitable for
组分
stable glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: no
glucose: high
NaHCO3: yes
运输
ambient
储存温度
2-8°C
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一般描述
该 DMEM-Hi 葡萄糖培养基与原始配方不同,因为它补充有L-丙氨酰-L-谷氨酰胺二肽。 众所周知,谷氨酰胺在细胞培养中不稳定。该二肽提供更稳定形式的谷氨酰胺用于细胞培养。它也不同于原始的DMEM-Hi 配方,其中吡哆醇代替吡哆醛。 吡哆醛是一种不稳定的培养基成分。
应用
Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。
法律信息
StableCell is a trademark of Sigma-Aldrich Co. LLC
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产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
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