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Merck
CN

D0819

Sigma-Aldrich

DMEM - high glucose

StableCell, with stable glutamine, and sodium bicarbonate, without sodium pyruvate, sterile liquid, suitable for cell culture

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别名:
杜氏改良 Eagle 培养基 - 高葡萄糖, Dulbecco’s Modified Eagle’s Medium, DME, DMEM
UNSPSC代码:
12352207
NACRES:
NA.75

product name

StableCell DMEM - 高葡萄糖, With 4500 mg/L glucose, stable glutamine, and sodium bicarbonate, without sodium pyruvate., liquid, sterile-filtered, suitable for cell culture

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

适用性

suitable for

组分

stable glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: no
glucose: high
NaHCO3: yes

运输

ambient

储存温度

2-8°C

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一般描述

该 DMEM-Hi 葡萄糖培养基与原始配方不同,因为它补充有L-丙氨酰-L-谷氨酰胺二肽。 众所周知,谷氨酰胺在细胞培养中不稳定。该二肽提供更稳定形式的谷氨酰胺用于细胞培养。它也不同于原始的DMEM-Hi 配方,其中吡哆醇代替吡哆醛。 吡哆醛是一种不稳定的培养基成分。

应用

Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。

法律信息

StableCell is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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