跳转至内容
Merck
CN
所有图片(3)

文件

安全信息

B9146

Sigma-Aldrich

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷

≥98%

登录查看公司和协议定价
所有图片(3)
别名:
5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, BCIG, X-Gal
经验公式（希尔记法）:
C14H15BrClNO6
CAS号:
7240-90-6
分子量:
408.63
Beilstein:
1402009
EC 号:
230-640-8
MDL编号:
MFCD00005666
UNSPSC代码:
12352204
PubChem化学物质编号:
57653968
NACRES:
NA.83

质量水平

200

检测方案

≥98%

形式

powder

溶解性

DMF: methanol (1:1): soluble 50 mg/mL

适用性

suitable for identification of lac+ bacterial colonies

储存温度

−20°C

SMILES字符串

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

正在寻找类似产品? 访问 产品对比指南

相关类别

应用

5-溴-4-氯-3-吲哚 β-D-半乳吡喃糖苷已被用于验证Caki-2/LacZ克隆的转导是否成功。它还可用于转化子的蓝白班筛选。
X-Gal是β-半乳糖苷酶的发色底物，可产生丰富的蓝色，与背景相比可以直观地检测到。 X-Gal是lac+基因型重组细菌菌落蓝白斑筛选的首选基质。

重悬

储备溶液（100 mg/ml）是通过将 X-Gal 溶解在二甲基甲酰胺中制备的，该溶液应避光储存在 −20 °C。无需灭菌。

相关产品

产品编号
说明
价格

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

HSV-tk gene therapy for human renal cell carcinoma in nude mice
Pulkkanen K, et al.
Cancer Gene Therapy, 8(7), 529-529 (2001)
Rolling circle amplification to screen yam germplasm for badnavirus infections and to amplify and characterise novel badnavirus genomes
Bomer M, et al.
Bio-protocol, 8(1), 529-529 (2018)
Enzo R Porrello et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(1), 187-192 (2012-12-19)
We recently identified a brief time period during postnatal development when the mammalian heart retains significant regenerative potential after amputation of the ventricular apex. However, one major unresolved question is whether the neonatal mouse heart can also regenerate in response
Khanh-Van Tran et al.
Cell metabolism, 15(2), 222-229 (2012-02-14)
Adipose tissue expansion involves the enlargement of existing adipocytes, the formation of new cells from committed preadipocytes, and the coordinated development of the tissue vascular network. Here we find that murine endothelial cells (ECs) of classic white and brown fat
Yibing Wang et al.
PloS one, 8(3), e59195-e59195 (2013-03-26)
Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (retaining a
查看所有相关文献

商品

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

细菌转化实验方案

感受态细胞生长及其转化（吸收DNA）的通用实验方案。

酵母转化简介

转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

实验方案

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

酵母转化实验方法

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

查看所有结果

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门