用途
sufficient for 200 tests
质量水平
包装
pkg of 1 kit
技术
flow cytometry: suitable
应用
cell analysis
detection
检测方法
fluorometric
运输
wet ice
储存温度
2-8°C
特点和优势
相比于TUNEL等基于DNA的检测方法，该方法能更早发现细胞凋亡进程。
- 快速标记细胞。只需10分钟即可完成细胞染色。
- 无需细胞固定或预处理，缩短检测时间并允许在后续研究中继续使用细胞。
- 附带6-羧基荧光素二醋酸盐二级染料，可从活细胞和坏死细胞中区分凋亡细胞。
原理
Sigma的Annexin V-Cy3试剂盒可以配合荧光显微镜来检测凋亡细胞上结合的膜联蛋白V。该试剂盒采用Cy3.18作为偶联膜联蛋白的荧光染料。通过显微镜检测发现，Cy3.18的荧光亮度比FITC偶联物更高。该试剂盒包含非荧光化合物6-羧基荧光素二醋酸盐 (6-CFDA)，后者可以进入到细胞并被活细胞中的酯酶水解，形成荧光化合物6-羧基荧光素，从而证明细胞是否存活。结合荧光显微镜，就可以区分出早期凋亡细胞（膜联蛋白V阳性，6-CFDA阳性）、坏死细胞（膜联蛋白V阳性，6-CFDA阴性）和活细胞（膜联蛋白V阴性，6-CFDA阳性）。
法律信息
Cy3 is a trademark of Cytiva
仅试剂盒组分
产品编号
说明
- Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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商品
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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