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Merck
CN

APOAC

Sigma-Aldrich

Annexin V-Cy3 细胞凋亡检测试剂盒

别名:

Red fluorescent Annexin V apoptosis assay

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.32

用途

sufficient for 200 tests

质量水平

包装

pkg of 1 kit

技术

flow cytometry: suitable

应用

cell analysis
detection

检测方法

fluorometric

运输

wet ice

储存温度

2-8°C

特点和优势

相比于TUNEL等基于DNA的检测方法,该方法能更早发现细胞凋亡进程。
  • 快速标记细胞。只需10分钟即可完成细胞染色。
  • 无需细胞固定或预处理,缩短检测时间并允许在后续研究中继续使用细胞。
  • 附带6-羧基荧光素二醋酸盐二级染料,可从活细胞和坏死细胞中区分凋亡细胞。

原理

Sigma的Annexin V-Cy3试剂盒可以配合荧光显微镜来检测凋亡细胞上结合的膜联蛋白V。该试剂盒采用Cy3.18作为偶联膜联蛋白的荧光染料。通过显微镜检测发现,Cy3.18的荧光亮度比FITC偶联物更高。该试剂盒包含非荧光化合物6-羧基荧光素二醋酸盐 (6-CFDA),后者可以进入到细胞并被活细胞中的酯酶水解,形成荧光化合物6-羧基荧光素,从而证明细胞是否存活。结合荧光显微镜,就可以区分出早期凋亡细胞(膜联蛋白V阳性,6-CFDA阳性)、坏死细胞(膜联蛋白V阳性,6-CFDA阴性)和活细胞(膜联蛋白V阴性,6-CFDA阳性)。

法律信息

Cy3 is a trademark of Cytiva

仅试剂盒组分

产品编号
说明

  • Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • C50416-Carboxyfluorescein diacetate 10 mg化学品安全说明书

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Scientific reports, 6, 32808-32808 (2016-09-07)
Metastasis of lung carcinoma to breast and vice versa accounts for one of the vast majority of cancer deaths. Synergistic treatments are proven to be the effective method to inhibit malignant cell proliferation. It is highly advantageous to use the

商品

细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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