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Merck
CN

A9165

Sigma-Aldrich

N-乙酰基-L-半胱氨酸

≥99% (TLC), suitable for cell culture, BioReagent

别名:

LNAC, NAC

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About This Item

线性分子式:
HSCH2CH(NHCOCH3)CO2H
CAS号:
分子量:
163.19
Beilstein:
1724426
EC 号:
MDL编号:
UNSPSC代码:
12352209
PubChem化学物质编号:
NACRES:
NA.26

product name

N-乙酰基-L-半胱氨酸, BioReagent, suitable for cell culture

产品线

BioReagent

质量水平

检测方案

≥99% (TLC)

形式

powder

技术

cell culture | mammalian: suitable

颜色

white to off-white

mp

106-108 °C (lit.)

溶解性

H2O: 100 mg/mL (with heating)

储存温度

2-8°C

SMILES字符串

CC(=O)N[C@@H](CS)C(O)=O

InChI

1S/C5H9NO3S/c1-3(7)6-4(2-10)5(8)9/h4,10H,2H2,1H3,(H,6,7)(H,8,9)/t4-/m0/s1

InChI key

PWKSKIMOESPYIA-BYPYZUCNSA-N

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应用

N-乙酰-L-半胱氨酸已被用作小鼠肠道干细胞培养的肠道基础培养基的组成部分,也被用作膨胀培养基的组成部分。

生化/生理作用

抗氧化剂和黏液溶解剂(mucolytic agent)。增加细胞池的自由基清除剂。 据报道可防止神经细胞凋亡,但会诱导平滑肌细胞凋亡。抑制HIV复制。可作为微粒体谷胱甘肽转移酶的底物。

通常也和此产品一起购买

产品编号
说明
价格

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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在文件库中查找您最近购买产品的文档。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. My media turned yellow when I added N-acetyl cysteine (NAC), Product A9165.  Why?

    The pH of a 1% solution is 2.0-2.8.  You may need to adjust the pH of the media with NaOH.  Or, prior to addition to the media, you can initially dilute the NAC with PBS, and then add it to the culture media.

  4. How stable is a solution of Product A9165, N-acetyl cysteine (NAC)?

    Aqueous solutions of cysteine oxidize to cystine on contact with air at neutral or alkaline pH. We recommend making aqueous solutions fresh.  NAC is relatively stable in acid.  Solutions in acid are stable and can be stored at -20 °C.

  5. What concentration of Product A9165, N-Acetyl-L-cysteine (NAC), should I use for cell culture?

    Cell culture testing is performed at 0.82 g/L.  The exact concentration will depend upon your application.

  6. What is the half life of Product A9165, N-Acetyl-L-cysteine (NAC), in cell culture?

    We have not tested the half-life of NAC solutions in our labs here at Sigma. However, this subject has been reviewed in Neuropsychopharmacology, 29, 2018-2023 (2004).

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Margherita Turco
Protocol Exchange, 2639-2644 (2017)
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
Kevin P. O?Rourke
Bio-protocol, 6(4), e1733-e1733 (2016)
Meritxell Huch et al.
Nature, 494(7436), 247-250 (2013-01-29)
The Wnt target gene Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon, stomach and hair follicles. A three-dimensional culture system allows long-term clonal expansion of single Lgr5(+) stem
Suzanne M Cloonan et al.
Journal of medicinal chemistry, 58(11), 4494-4505 (2015-05-12)
Ruthenium polypyridyl complexes show great promise as new photodynamic therapy (PDT) agents. However, a lack of detailed understanding of their mode of action in cells poses a challenge to their development. We have designed a new Ru(II) PDT candidate that
Yuval Samuni et al.
Biochimica et biophysica acta, 1830(8), 4117-4129 (2013-04-27)
N-acetylcysteine (NAC) has been in clinical practice for several decades. It has been used as a mucolytic agent and for the treatment of numerous disorders including paracetamol intoxication, doxorubicin cardiotoxicity, ischemia-reperfusion cardiac injury, acute respiratory distress syndrome, bronchitis, chemotherapy-induced toxicity

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