Quality Level
packaging
pkg of 1 kit
storage condition
protect from light
fluorescence
λex 490 nm; λem 502 nm (PKH67 dye)
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
Application
用于常规细胞膜标记的PKH26红色荧光细胞连接子试剂盒已用于：
- 标记并进一步研究在三阴性乳腺癌状况下从细胞中排出的外泌体。
- 标记凋亡性细胞，用于研究ανβ5受体在凋亡性细胞的结合和内化中的作用。
- 在荧光成像中。
本试剂盒适用于常规细胞膜标记。PKH67具有长于PKH1和PKH2的脂肪炭尾，后两者是另外两种之前常用于体外和体内细胞示踪的绿色染料。由于炭尾长度更长，内部研究已经证明PKH67比PKH2由更少的细胞间转移。
在采用PKH1和PKH2进行的体内研究中，荧光强度都会缓慢损失。由于这是绿色细胞linker染料而非红色细胞linker染料出现的行为特征，因而PKH67会出现类似的性质。不分裂细胞的体外细胞膜留存和体内荧光半衰期的关联性揭示，PKH67的体内荧光半衰期为10-12天。其他具有类似半衰期的绿色细胞linker染料已经被用于监测1-2月内的体内 淋巴细胞和巨噬细胞运输，结果表明PKH67还可用于中等时长的体内跟踪研究。
在采用PKH1和PKH2进行的体内研究中，荧光强度都会缓慢损失。由于这是绿色细胞linker染料而非红色细胞linker染料出现的行为特征，因而PKH67会出现类似的性质。不分裂细胞的体外细胞膜留存和体内荧光半衰期的关联性揭示，PKH67的体内荧光半衰期为10-12天。其他具有类似半衰期的绿色细胞linker染料已经被用于监测1-2月内的体内 淋巴细胞和巨噬细胞运输，结果表明PKH67还可用于中等时长的体内跟踪研究。
Other Notes
如需关于PKH及CellVue®的荧光细胞连接子染料的更多技术详情，包括大量的参考书目，请访问这里。
Legal Information
CellVue is a registered trademark of Phanos Technologies
仅试剂盒组分
产品编号
说明
- Diluent C 6 x 10
- PKH67 Cell Linker in ethanol .5 mL
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
存储类别
3 - Flammable liquids
flash_point_f
57.2 °F - closed cup
flash_point_c
14 °C - closed cup
法规信息
危险化学品
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| PKH67GL-1KT | 04061836680947 |