活细胞成像试剂

实时的活细胞成像可使科学家能够对无法使用传统的终点技术（例如固定成像和PCR仅能捕获一个静态的时刻）观察的动态细胞事件进行监测。我们产品的活细胞成像试剂组合包括用于细胞示踪的新型荧光细胞标记技术、荧光慢病毒生物传感器、传统荧光染料和用于实时分析细胞事件的探针。这些试剂适于通过CellASIC^® ONIX2微流体活细胞成像系统等实时孵育和成像系统捕获动态细胞事件。

概要

PKH和CellVue^®荧光细胞连接子标记物
LentiBrite™荧光慢病毒生物传感器
BioTracker活细胞荧光染料和染色剂
LuminiCell Tracker™荧光纳米粒子

PKH和 CellVue^®荧光细胞连接子标记

PKH和CellVue^®细胞荧光连接试剂盒可为长时间的活细胞成像实验提供活细胞荧光膜标记。

PKH和CellVue^®染料主要特点：

对体内活细胞稳定长达100天
对于真核细胞、细菌细胞和寄生虫是安全的
无明显的染料漏出或细胞间转移
信号均匀且可重现
利用光谱不同的染料可实现多色分析
兼容其他荧光标记物
已被7,000多份出版物所引用

LentiBrite™荧光慢病毒生物传感器

LentiBrite™荧光慢病毒生物传感器是一套预包装的慢病毒颗粒，编码自噬、凋亡和细胞结构相关荧光蛋白，使活细胞中的这些过程分析可视化。

慢病毒生物传感器的特点：

预包装、即用型形式、标记了单体GFP和RFP
每管最低滴度（≥3 x 108 IFU/mL）
转染效率优于化学方法
适用于分裂中、未分裂以及难转染的细胞，如原代细胞或干细胞
不破坏细胞功能

BioTracker活细胞荧光染料和染色剂

BioTracker活细胞染料是一类细胞可渗透性染料，适用于细胞器标记、细胞凋亡检测、细胞活力和细胞健康分析、缺氧监测、活性氧示踪、钙指示剂功能以及神经细胞和干细胞(SCR150, SCT029, SCT046)培养等活细胞应用。

LuminiCell Tracker™荧光纳米粒子

LuminiCell Tracker™ 荧光纳米粒子为长期的细胞追踪实验提供了一种有效、简单、无毒的试剂。  这种细胞追踪染料具有聚集诱导发光(AIE)特性，封装在生物相容性、细胞可渗透的荧光纳米粒子内（AIE纳米点），可提供增强的光稳定性、超亮荧光和低细胞毒性，适用于较长时间的生物成像应用。

荧光更强：是很多细胞标记技术的10倍
光稳定性：荧光信号不淬灭的时间延长3倍
生物相容性：无毒性有机纳米粒子适合生物学应用
快速标记：简单易行的4小时内细胞标记

相关视频

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使用CellASIC<sup>®</sup> ONIX2微流控系统对缺氧癌细胞进行活细胞成像

使用CellASIC^® ONIX2微流控系统对缺氧癌细胞进行活细胞成像

A431 epidermal carcinoma cells (85090402) were incubated 16 hours overnight with 3µM BioTracker 520 Green Hypoxia Dye (SCT033) in complete DMEM by gravity perfusion at 37°C under normoxic conditions (20% O2) using the CellASIC® ONIX2 System. After overnight incubation, cells were perfused with complete DMEM media without dye at 1 psi under hypoxic conditions (0% O2) for 16 hours at 37°C. Fluorescent images were captured over 16 hours using 465 nm excitation, and a 525 nm emission filter. The BioTracker 520 Green Hypoxia Dye increases in fluorescence intensity with decreasing oxygen levels (increased hypoxic conditions).

Live Cell Imaging of p62 using LentiBrite™ Fluorescent Biosensors

Human mesenchymal stem cells (MSCs), were plated in a chamber slide and transduced with LentiBrite™ RFP-p62 lentiviral particles at an MOI of 40 for 24 hours. After media replacement and 24 hours further incubation, cells were incubated for 4 hours in EBSS containing a lysosome inhibitor, to induce autophagy and inhibit lysosomal degradation of autophagosomes.

Live Cell Imaging of β-Actin Cytoskeleton Proteins using LentiBrite™ Fluorescent Biosensors

U2OS cells were plated in a chamber slide and transduced with LentiBrite™ GFP-β-actin lentiviral particles at an MOI of 20 for 24 hours. After media replacement and an additional 48 hours of further incubation, the cells were treated with 2 µM cytochalasin-D for another 2 hours, to induce actin depolymerization. The GFP- β -actin displays a filamentous distribution in the untreated cells, and upon actin depolymerization, adopts a punctate distribution. LentiBrite™ Lentiviral Biosensors are a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.

Live Cell Imaging of Autophagy using LentiBrite™ Fluorescent Biosensors

HT-1080 cells were plated in a chamber slide and transduced with LentiBrite™ GFP-LC3 lentiviral particles at an MOI of 20 for 24 hours. After media replacement and 48 hours further incubation, cells were incubated for 4 hours in EBSS containing a lysosome inhibitor, to induce autophagy and inhibit lysosomal degradation of autophagosomes. LentiBrite™ Lentiviral Biosensors are a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.

Live Cell Imaging of Apoptosis in Immune Cells using the CellASIC® ONIX2 Microfluidic System

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of different cell types in vitro and in vivo.