质量水平
包装
pkg of 1 kit
储存条件
protect from light
荧光
λex 490 nm; λem 502 nm (PKH67 dye)
检测方法
fluorometric
运输
ambient
储存温度
room temp
应用
在采用PKH1和PKH2进行的体内研究中，荧光强度都会缓慢损失。由于这是绿色细胞linker染料而非红色细胞linker染料出现的行为特征，因而PKH67会出现类似的性质。不分裂细胞的体外细胞膜留存和体内荧光半衰期的关联性揭示，PKH67的体内荧光半衰期为10-12天。其他具有类似半衰期的绿色细胞linker染料已经被用于监测1-2月内的体内 淋巴细胞和巨噬细胞运输，结果表明PKH67还可用于中等时长的体内跟踪研究。
- 标记并进一步研究在三阴性乳腺癌状况下从细胞中排出的外泌体。
- 标记凋亡性细胞，用于研究ανβ5受体在凋亡性细胞的结合和内化中的作用。
- 在荧光成像中。
联系
法律信息
仅试剂盒组分
- Diluent C 6 x 10
- PKH67 Cell Linker in ethanol .5 mL
警示用语：
Danger
危险声明
危险分类
Eye Irrit. 2 - Flam. Liq. 2
储存分类代码
3 - Flammable liquids
闪点(°F)
57.2 °F - closed cup
闪点(°C)
14 °C - closed cup
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Does the dye leak from the cells after labeling when using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling?
In general, slow loss of fluorescence has been observed with PKH1 and PKH2 in in vivo studies and it is likely that PKH67 may also slowly lose fluorescence. Leakage (or loss of staining intensity) may also occur due to cell-to-cell transfer of the dye. The most common cause of cell-to-cell dye transfer is inadequate washing of the cells. Wash cells 3-5 times after labeling and transfer samples to new tubes between washes.
When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?
Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.
Are cells lost during the PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling staining process?
Over-labeling of the cells will result in loss of membrane integrity and reduced cell recovery. Methods for improving cell viability can be found on the troubleshooting guide.
What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?
A protocol for visualization of tissue sections can be found in the technical bulletin. For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells. If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the difference between Green Fluorescent Cell Linker Kits PKH2 and PKH67?
PKH2 was one of the early PKH dyes. The PKH67 dye has a longer aliphatic tail. There is reduced cell-celldye transfer for PKH67 as compared with PKH2.
How many cells can be stained with Product PKH67GL, Green Fluorescent Cell Linker Kit?
This kit can stain 50 × 107 cells if used as directed (1 × 107 cells stained with 2 × 10-6 M PKH67).
What is the excitation and emission spectra for Product PKH67GL, Green Fluorescent Cell Linker Kit?
The spectra can be found on the product insert.The product has a maximum excitation at 490 nm and maximum emission at 502 nm.
Will Product PKH67, Green Fluorescent Cell Linker Kit, stain dead cells?
As long as the cell has an intact membrane, the PKH76 dye can label the cell.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
默克提供用于细胞荧光标记的PKH LINKER试剂盒，PKH荧光细胞接头试剂盒使用获得专利的荧光细胞接头技术将报告分子结合到细胞膜中。
PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.
Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.
亲脂性细胞追踪染料使癌症生物学家能够在体外和体内追踪肿瘤和免疫细胞功能。阅读文章，选择适合细胞追踪和增殖监测的膜染料试剂盒。
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