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MIDI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Synonym(s):

Green PKH membrane midi labeling kit

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100 G
CN¥2,991.31

CN¥2,991.31

Available to ship onApril 09, 2025Details

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100 G
CN¥2,991.31

About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

CN¥2,991.31

Available to ship onApril 09, 2025Details

packaging

pkg of 1 kit

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

technique(s)

flow cytometry: suitable

fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

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Show Differences

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This Item
B7535B4429B9754
BIS-TRIS BioUltra, ≥99.0% (NT)

14879

BIS-TRIS

BIS-TRIS BioXtra, ≥98.0% (titration)

B7535

BIS-TRIS

BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

B4429

BIS-TRIS

BIS-TRIS ≥98.0% (titration)

B9754

BIS-TRIS

Quality Level

200

Quality Level

400

Quality Level

400

Quality Level

400

product line

BioUltra

product line

BioXtra

product line

-

product line

-

form

powder

form

powder

form

crystalline powder

form

crystalline powder

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

H2O: 1 M, clear, colorless

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

pH

9.5-11.0 (25 °C, 1 M in H2O)

pH

9.0-10.1 (20-25 °C, 1 M in H2O)

pH

-

pH

-

General description

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Application

PKH67 Green Fluorescent Cell Linker Midi Kit has been used for:
  • labeling exosomes[1]  
  • extracellular vesicles (EVs)[2]l
  • normal and sickle red blood cells (RBCs)[3]
  • Raji cells[4]

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

CellVue is a registered trademark of Phanos Technologies

Kit Components Only

Product No.
Description
  • PKH67 Dye 2 x 0.1
  • Diluent C 6 x 10

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14 °C - closed cup

Regulatory Information

危险化学品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    MKCT9211
    MKCQ8263
    MKCP3716
    MKCL5579
    MKCK8898

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    Find documentation for the products that you have recently purchased in the Document Library.

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    ADCC Assay Protocol
    Fang L and He F
    Journal of Virology (2013)
    Modulation of Sickle Red Blood Cell Adhesion and its Associated Changes in Biomarkers by Sulfated Nonanticoagulant Heparin Derivative
    Alshaiban A, et al.
    Clinical and Applied Thrombosis/Hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 22, 230-238 (2016)
    Notochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells
    Bach F, et al.
    Testing, 8(51), 88845-88845 (2017)
    Dongxin Tang et al.
    Clinical and translational medicine, 10(4), e139-e139 (2020-09-09)
    Cancer stem cells (CSCs) are important factors contributing to tumorigenesis. We examined whether CSCs isolated from colorectal cancer (CRC) cells possess metastatic properties that can be transferred to non-CSCs via the delivery of miR-200c enclosed in extracellular vesicles (EVs). The
    Chuang Gao et al.
    Aging, 11(24), 12147-12164 (2019-12-17)
    Exosomes are small (30-150 nm diameter) lipid bilayer-enclosed vesicles found in all bodily fluids. We investigated whether exosomes play a role in chronic subdural hematoma (CSDH). Exosomes were identified and characterized using transmission electron microscopy and NanoSight particle tracking. The
    View All Related Papers

    Articles

    Cell Tracking with Lipophilic Membrane Dyes

    Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

    PKH and CellVue® Claret Fluorescent Cell Linker Dyes

    PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service