This kit is for phagocytic cell labeling. It is used to selectively label cells with phagocytic capabilities such as monocytes, macrophages or neutrophils.

Biochem/physiol Actions

The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for at least 21 days in vivo.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.

Other Notes

For additional technical details on PKH and CellVue^® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Legal Information

CellVue is a registered trademark of Phanos Technologies

pictograms

GHS02,GHS07

signalword

Danger

hcodes

H225,H319

pcodes

P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

flash_point_f

57.2 °F - closed cup

flash_point_c

14.0 °C - closed cup

Regulatory Information

危险化学品

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Tumor growth and metastasis suppression by Glipr1 gene-modified macrophages in a metastatic prostate cancer model.

K Tabata et al.

Gene therapy, 18(10), 969-978 (2011-04-23)

We previously identified the mouse and human Glipr1 and GLIPR1/RTVP-1 genes, respectively, as direct p53 targets with proapoptotic activities in various cancer cell lines, including prostate cancer (PCa). Intratumoral injection of an adenoviral vector capable of efficient transduction and expression

The neovascularization effect of bone marrow stromal cells in temporal muscle after encephalomyosynangiosis in chronic cerebral ischemic rats.

Hyung-Syup Kim et al.

Journal of Korean Neurosurgical Society, 44(4), 249-255 (2008-12-20)

In Moyamoya disease, the primary goal of treatment is to improve collateral circulation through angiogenesis. In the present study, we obtained and sub-cultured bone marrow stromal cells (BMSCs) from rats without a cell-mediated immune response. Then, we injected the labeled

The prolonged life-span of alveolar macrophages.

Jaime Murphy et al.

American journal of respiratory cell and molecular biology, 38(4), 380-385 (2008-01-15)

To further examine the half-life of alveolar macrophages, chimeric CD 45.2 mice were generated through bone marrow transplantation of donor CD 45.1 cells. Before administration of donor cells, recipient mice were divided into two cohorts: the first cohort received total

Single-cell analysis reveals new subset markers of murine peritoneal macrophages and highlights macrophage dynamics upon Staphylococcus aureus peritonitis.

Solène Accarias et al.

Innate immunity, 22(5), 382-392 (2016-05-26)

Resident macrophages play a central role in maintaining tissue homeostasis and immune surveillance. Here, we used single cell-based qPCR coupled with flow cytometry analysis to further define the phenotypes of large and small resident peritoneal macrophages (LPMs and SPMs, respectively)

Migration of neutrophils targeting amyloid plaques in Alzheimer's disease mouse model.

Sung Hoon Baik et al.

Neurobiology of aging, 35(6), 1286-1292 (2014-02-04)

Immune responses in the brain are thought to play a role in disorders of the central nervous system, but an understanding of the process underlying how immune cells get into the brain and their fate there remains unclear. In this

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PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling

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