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Merck
CN
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文件

安全信息

19160

Sigma-Aldrich

超氧化物歧化酶(SOD)检测试剂盒

sufficient for 500 tests

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别名:
超氧化物歧化酶检测试剂盒
UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 500 tests

质量水平

100

存货情况

not available in USA

技术

UV/Vis spectroscopy: suitable

适用性

corresponds for UV test (WST reagent, WST formazan)
use tested

储存温度

2-8°C

相关类别

一般描述

该试剂盒含有所有测定超氧化物歧化酶活性所需的试剂和溶液，使用了基于黄嘌呤氧化酶和全新显色试剂的间接分析方法。在该试剂盒中所用的显色试剂的化学及生物化学特性保证了应用的便利性和测试结果在宽范围内的线性。

应用

SOD检测试剂已用于在哺乳动物细胞中对超氧化物歧化酶的活性进行测定。

生化/生理作用

超氧化物歧化酶是负责消除超氧自由基（O2-）的酶，从而可保护细胞免受自由基相关的损伤。他们可催化两个超氧化物自由基歧化为过氧化氢和氧气。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is contained in Product 19160, SOD Assay Kit (Superoxide dismutase)?

    This kit contains WST Solution (1x5mL), Enzyme Solution (1x100μL), Buffer Solution (1x100mL), Dilution Buffer (1x50mL), and a manual.  

  4. What equipment is required to use Product 19160, SOD Assay Kit (Superoxide dismutase)?

    The equipment needed to use this kit is a plate reader (450 nm filter), a 96-well microplate, 10 μL & 100-200 μL pipettes, a multichannel pipette and an incubator. Superoxide dismutase (SOD) is also necessary for the preparation of an inhibition curve.

  5. How do I prepare a WST working solution for Product 19160, SOD Assay Kit (Superoxide dismutase)?

    Dilute 1mL of WST Solution with 19mL of Buffer Solution.

  6. When using Product 19160, SOD Assay Kit (Superoxide dismutase), how do I prepare an Enzyme working solution?

    Centrifuge the Enzyme Solution tube for five seconds.  Mix by pipeting, and dilute 15μL of Enzyme Solution with 2.5mL of Dilution Buffer.

  7. When using Product 19160, SOD Assay Kit, how do I prepare a Superoxide Dismutase (SOD) working solution?

    Dilute SOD with Dilution Buffer to prepare SOD standard Solutions if necessary for assay monitoring as follows:  200 U/mL, 100 U/mL, 50 U/mL, 20 U/mL, 10 U/mL, 5 U/mL, 1 U/mL, 0.1 U/mL, 0.05 U/mL, 0.01 U/mL, and 0.001 U/mL.

  8. Are there any literature references for Product 19160, SOD Assay Kit (Superoxide dismutase)?

    1. J. M. McCord and I. Fridovich, J. Biol. Chem., 244, 6049 (1969).  2. S. Goldstein and G. Czapski, Free Rad. Res. Comms.,12-13(Part 1), 5-10 (1991).  3. R. H. Burdon, V. Gill, and C. Rice-Evans, Free Rad. Res. Comms., 18, 369 (1993).  4. M. W. Sutherland and B. Learmonth, Free Rad. Res., 27, 283 (1997).  5. H. Ukeda, A. K. Sarker, D. Kawana and M. Sawamura, Anal. Sci., 15, 353 (1999).  6. H. Ukeda, D. Kawana, S. Maeda and M. Sawamura, Biosci. Biotechnol. Biochem., 63, 485 (1999).  7. A. V. Peskin, et al., Clin. Chim. Acta., 293, 157 (2000).

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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