Application
适于研究各种组织和亚细胞器中的过氧化氢酶活性。
适用于比色法和紫外分光光度法检测
Biochem/physiol Actions
该测定方法旨在测定过氧化氢酶作用后残留的过氧化氢底物。首先，过氧化氢在过氧化氢酶作用下转化为水和氧气（过氧化氢酶途径），然后通过叠氮化钠终止这一酶促反应。随后通过比色法，分析反应混合物等分试样中余下的过氧化氢量。10 比色法使用取代苯酚（3,5-二氯-2-羟基苯磺酸），在存在过氧化氢和辣根过氧化物酶（HRP）的条件下，通过氧化作用偶联至4-氨基安替比林中，生成吸收波长520 nm的红色醌亚胺染料（N-（4-安替比林基）-3-氯-5-磺酸酯对苯醌单亚胺）。
过氧化氢酶是一种普遍存在的抗氧化酶，其功能是催化过氧化氢(H2O2) 的分解，生成水和氧气。过氧化氢在真核细胞内以多种氧化酶和超氧化歧化酶的副产物形式存在。过氧化氢在细胞内的聚集会引发细胞内DNA，蛋白质和脂质的氧化，从而导致基因突变和细胞死亡。过氧化氢酶可去除细胞内的H2O2，保护活细胞免受氧化损伤，过氧化氢酶在氧化胁迫相关疾病中的作用已得到了广泛研究。
Features and Benefits
- 用于检测过氧化氢酶活性 - 可能用于各种不同组织和细胞
- 简单，优化的实验步骤 - 简单的比色法检测过氧化氢酶体富集和过氧化氢酶活性
General description
过氧化氢酶活性检测试剂盒包含研究多种组织和亚细胞组织中过氧化氢酶所需的所有组分。
Other Notes
在pH 7.0，25℃，过氧化氢底物浓度50mM条件下，一单位过氧化氢酶每分钟可以分解 1μmol 过氧化氢生成氧气和水。
Preparation Note
使用超纯水配置所有溶液。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
动植物源性产品
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M B Hampton et al.
FEBS letters, 414(3), 552-556 (1997-10-10)
The induction of apoptosis in Jurkat T-lymphocytes with 50 microM hydrogen peroxide was associated with caspase activation. Caspase activity was first detected 3 h after treatment, and the morphological features of apoptosis were apparent by 6 h. At higher concentrations
M Zámocký et al.
Progress in biophysics and molecular biology, 72(1), 19-66 (1999-08-14)
This review gives an overview about the structural organisation of different evolutionary lines of all enzymes capable of efficient dismutation of hydrogen peroxide. Major potential applications in biotechnology and clinical medicine justify further investigations. According to structural and functional similarities
S Tada-Oikawa et al.
FEBS letters, 442(1), 65-69 (1999-01-29)
Pulsed field gel electrophoresis showed that the initiation time of DNA breakage induced by the DNA alkylating agent duocarmycin A, which is not a redox-cycling agent, was almost the same in the human leukemia cell line HL-60 and its H2O2-resistant
M Ding et al.
Journal of cell science, 113 ( Pt 13), 2409-2419 (2000-06-15)
The intracellular protozoan parasite Toxoplasma gondii, like all members of the phylum Apicomplexa, is known to possess many organelles: in addition to mitochondria and the compartments of the secretory pathway, there is a reduced chloroplast (the apicoplast) and the phylum-specific
Susmita Das Nishu et al.
PloS one, 14(3), e0213370-e0213370 (2019-03-13)
Algicidal bacteria have received broad acceptance as an ecofriendly tool for controlling harmful algal blooms. However, their practical application is still limited to the lab-scale tests due to the complex alga-bacterium interactions in different nutrient statuses. In this study, the
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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
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Instructions
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