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无菌性
non-sterile
质量水平
表单
powder
保质期
limited shelf life, expiry date on the label
制造商/商品名称
NutriSelect® Plus
技术
microbiological culture: suitable
pH值(酸碱度)
7.8±0.2 (25 °C)
应用
clinical testing
food and beverages
microbiology
储存温度
2-25°C
适用性
nonselective for Streptococcus spp.
一般描述
Todd Hewitt Broth is used for cultivation of haemolytic streptococci for different serological tests. This medium has been recommended as an alternative type in epidemiologic studies of group A streptococci as well as pathogenic microorganisms. Todd Hewitt Broth medium is very nutritive by the presence of peptic digest of animal tissue and beef heart infusion. Dextrose stimulates haemolysin production. The medium is well buffered by sodium phosphate and sodium carbonate to neutralize the acid produced during dextrose fermentation. This restricts destruction of antigenic streptococcal haemolysin. It is also found that sodium phosphate has a stimulating effect on the pneumococcal growth. Todd Hewitt Broth can be employed as an alternative to serum broth or horse flesh digest broth for the cultivation of streptococci prior to serological typing.
应用
Recommended for the cultivation of group A hemolytic Streptococci used for serological studies.
Todd Hewitt broth has been used for the culture of various microorganisms, such as Bacillus subtilis and Streptococcus pneumoniae.
组分
组分 (g/L):
牛心浸粉 500.00
动物组织消化物 20.00
右旋糖 2.00
氯化钠 2.00
磷酸钠 0.40
碳酸钠 2.50
牛心浸粉 500.00
动物组织消化物 20.00
右旋糖 2.00
氯化钠 2.00
磷酸钠 0.40
碳酸钠 2.50
制备说明
Suspend 37 g of Todd Hewitt Broth in 1000 ml of distilled water. Mix well and dispense as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
附注
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法律信息
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type N95 (US)
法规信息
动植物来源培养基
The Journal of Pathology and Bacteriology, 35, 1-97 (1932)
Assessing membrane fluidity and visualizing fluid membrane domains in bacteria using fluorescent membrane dyes
Bio-protocol (2018)
Observation of Pneumococcal Phase Variation in Colony Morphology
Bio-protocol, 7(15) (2017)
American Journal of Public Health, 53, 1083-1083 (1963)
The pore-forming protein gasdermin D regulates interleukin-1 secretion from living macrophages
Immunity, 48(1), 35-44 (2018)
商品
链球菌 — 检测、鉴定、分化和培养技术概述
Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques
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