无菌性
non-sterile
质量水平
产品线
BioChemika
形式
powder
保质期
limited shelf life, expiry date on the label
制造商/商品名称
NutriSelect® Plus
储存条件
protect from light at room temperature
技术
microbe id | susceptibility testing: suitable
microbiological culture: suitable
最终pH
6.6±0.2 (25 °C)
应用
clinical testing
food and beverages
life science and biopharma
veterinary
microbiology
适用性
mycobacteria
一般描述
Middlebrook 7H11 Agar Base media is used for the isolation, cultivation, and sensitivity testing of Mycobacterium species. This agar base contains many inorganic salts which help the growth of Mycobacteria. Citric acid formed from sodium citrate helps in retaining inorganic cations in solution. Glycerol supplies carbon and energy. The Middlebrook OADC Supplement (M0678) contains oleic acid, bovine albumin, sodium chloride, dextrose and catalase. Oleic acid and other long chain fatty acids are essential for the metabolism of Mycobacteria. Dextrose is an energy source. Catalase neutralizes toxic peroxides. Albumin protects tubercle bacilli from toxic agents. Malachite green partially inhibits other bacteria.
应用
There are two types of solid culture media for the primary isolation of Mycobacteria. Egg-based (Lowenstein’s formulation) and agar-based (Middlebrook formulation)
The latter has the following advantages over the egg-based media
The Middlebrook 7H11 agar base does not liquify easily in the presence of contaminating proteolytic organisms
There is less inactivation of added drugs when egg ingredients are absent
The latter has the following advantages over the egg-based media
The Middlebrook 7H11 agar base does not liquify easily in the presence of contaminating proteolytic organisms
There is less inactivation of added drugs when egg ingredients are absent
组分
成分 (g/L)
酶水解酪蛋白，1.00
硫酸铵，0.50
磷酸二氢钾，1.50
磷酸二钠，1.50
柠檬酸钠，0.40
硫酸镁，0.05
L-谷氨酸，0.50
柠檬酸铁铵，0.04
吡哆素，0.001
生物素，0.0005
孔雀绿，0.001
琼脂，15.00
酶水解酪蛋白，1.00
硫酸铵，0.50
磷酸二氢钾，1.50
磷酸二钠，1.50
柠檬酸钠，0.40
硫酸镁，0.05
L-谷氨酸，0.50
柠檬酸铁铵，0.04
吡哆素，0.001
生物素，0.0005
孔雀绿，0.001
琼脂，15.00
制备说明
Suspend 10.25 g of Middlebrook 7H11 Agar Base in 450 ml of distilled water. Add 2.5 ml of glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 1 vial Middlebrook OADC Growth Supplement (M0678). Mix thoroughly before dispensing.
附注
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法律信息
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Shuxin Liang et al.
Autophagy, 16(8), 1453-1467 (2019-11-13)
BAG2 (BCL2 associated athanogene 2) is associated with cell fate determination in response to various pathological conditions. However, the effects of BAG2 on M. tuberculosis-induced endoplasmic reticulum (ER) stress remain elusive. Herein, we report that M. tuberculosis infection of macrophages
Thomas R Lerner et al.
The Journal of clinical investigation, 126(3), 1093-1108 (2016-02-24)
In extrapulmonary tuberculosis, the most common site of infection is within the lymphatic system, and there is growing recognition that lymphatic endothelial cells (LECs) are involved in immune function. Here, we identified LECs, which line the lymphatic vessels, as a
Antony Fearns et al.
PLoS biology, 18(12), e3000879-e3000879 (2021-01-01)
Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed correlative light, electron
MacFaddin, J
Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, 1 (1985)
商品
Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门