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Merck
CN
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安全信息

M0178

Millipore

Middlebrook 7H9 肉汤基础

suitable for microbiology, NutriSelect® Plus,

Recommended for use in isolation and cultivation of Mycobacterium species

别名:

Mycobacterial culture media

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About This Item

UNSPSC代码:
41171607
NACRES:
NA.85

质量水平

200

无菌性

non-sterile

产品线

BioChemika

形式

powder

保质期

limited shelf life, expiry date on the label

制造商/商品名称

NutriSelect® Plus

技术

microbe id | susceptibility testing: suitable
microbiological culture: suitable

最终pH

6.6±0.2 (25 °C)

应用

clinical testing
food and beverages
life science and biopharma
veterinary
microbiology

储存温度

2-25°C

适用性

mycobacteria

相关类别

一般描述

Middlebrook 7H9 Broth Base contains inorganic compounds that supply the elements potassium, sulfur, magnesium, and phosphorus which are required for appropriate growth of Mycobacteria. This medium with Middlebrook ADC Growth Supplement is recommended for ideal growth of Mycobacterium species.
Middlebrook ADC Growth Supplement (Product No. M0553) contains bovine albumin, dextrose, and catalase. Dextrose supplies energy, while albumin protects Mycobacteria from toxic agents and catalase splits toxic radicals.
Mycobacteria grow most rapidly in broth media and hence primary isolation of bacterial species can be carried out in Middlebrook 7H9 Broth Base.

应用

Middlebrook 7H9 Broth Base has been used for the cultivation Mycobacterium bovis bacillus Calmette-Guérin Pasteur strain. It is also recommended for cultivation and sensitivity testing of Mycobacterium tuberculosis.

组分

成分 (g/L)
硫酸铵，0.50
磷酸二钠，2.50
磷酸二氢钾，1.00
氯化钠，0.10
硫酸镁，0.05
氯化钙，0.0005
硫酸锌，0.001
硫酸铜，0.001
柠檬酸铁铵，0.04
L-谷氨酸，0.50
吡哆素，0.001
生物素，0.0005

制备说明

Suspend 2.35 g of Middlebrook 7H9 Broth Base in 450 ml of distilled water. Add either 2 ml of glycerol (Cat. No 49767) or 0.5 g of TWEEN® 80 (Cat. No. 93780). Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. Pressure (121°C) for 10 minutes. Cool to 45°C or below and aseptically add 1 vial of Middlebrook ADC Growth Supplement (Cat. No. M0553). Mix well before dispensing.

附注

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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分析证书(COA)

Lot/Batch Number

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Differential requirements for L-citrulline and L-arginine during antimycobacterial macrophage activity
Rapovy SM, et al.
Journal of Immunology, 195(7), 3293-3300 (2015)
Peter Masoko et al.
Evidence-based complementary and alternative medicine : eCAM, 2013, 586247-586247 (2013-08-21)
The aim of the study was to scientifically evaluate the antimycobacterial activity of selected indigenous medicinal plants from the Limpopo Province used for the treatment of humans with symptoms of Mycobacterium tuberculosis. The leaves of five plant species (Apodytes dimidiata
l-Citrulline Metabolism in Mice Augments CD4+ T Cell Proliferation and Cytokine Production In Vitro, and Accumulation in the Mycobacteria-Infected Lung
Lange S M, et al.
Frontiers in Immunology, 8, 1561-1561 (2017)
Nancy Patience Motlalepula Komape et al.
BMC complementary and alternative medicine, 17(1), 128-128 (2017-02-27)
Tuberculosis is an infectious communicable disease and the causative agent of the disease has over the years developed resistance to streamline chemotherapeutic agents with dire consequences and there is a need for development of new and more potent alternatives. Constituents
Daniel J Greenwood et al.
Science (New York, N.Y.), 364(6447), 1279-1282 (2019-06-30)
Tuberculosis, caused by the intracellular pathogen Mycobacterium tuberculosis, remains the world's deadliest infectious disease. Sterilizing chemotherapy requires at least 6 months of multidrug therapy. Difficulty visualizing the subcellular localization of antibiotics in infected host cells means that it is unclear
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商品

Detection & Molecular Identification of Mycobacteria

Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.

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