跳转至内容
Merck
CN
所有图片(8)

文件

E7750

Sigma-Aldrich

N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺 盐酸盐

commercial grade, powder

登录查看公司和协议定价
所有图片(8)
别名:
N-乙基-N′ -（3-二甲基氨基丙基）碳二亚胺 盐酸盐, EDAC, EDC, EDC 盐酸盐, WSC 盐酸盐
经验公式（希尔记法）:
C8H17N3 · HCl
CAS号:
25952-53-8
分子量:
191.70
Beilstein:
5764110
EC 号:
247-361-2
MDL编号:
MFCD00012503
UNSPSC代码:
12352111
PubChem化学物质编号:
24894654
NACRES:
NA.31

质量水平

300

等级

commercial grade

形式

powder

技术

Northern blotting: suitable
bioconjugation: suitable

颜色

white to off-white

mp

110-115 °C (lit.)

溶解性

H2O: ≤100 mg/mL

储存温度

−20°C

SMILES字符串

Cl.CCN=C=NCCCN(C)C

InChI

1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H

InChI key

FPQQSJJWHUJYPU-UHFFFAOYSA-N

正在寻找类似产品? 访问 产品对比指南

一般描述

1-乙基-3-（3-二甲基氨基丙基）碳酰二亚胺盐酸盐（EDC HCl）常被称为EDAC，它是化学和生物化学研究领域功能强大、应用广泛的水溶性试剂，在促进酰胺键的形成中起关键作用。在肽合成领域，EDC HCl高效催化羧基和胺基与氨基酸的偶联，从而促进形成具有特定序列和功能的肽骨架（peptide backbone）。这种特性在各种研究用肽的生产中有重要作用。除了肽以外，EDC HCl的影响还扩展到了免疫原的构建，它主动参与半抗原（诱发免疫反应的小分子）与载体蛋白的共价连接。这种参与有助于疫苗的研究和开发。

EDC HCl的多功能性还体现在其修饰核酸的能力，从而通过其5′磷酸基团标记DNA和RNA。这种功能有助于这些关键分子的成像、追踪和分析，为核酸研究的进步做出重大贡献。此外，EDC HCl可充当重要的生物分子桥（ biomolecule bridge），它作为交联剂将生物分子中的胺反应性NHS酯与羧基连接起来。这种技术在蛋白质偶联中有重要价值，可以创造出具有全新特性和功能的杂交分子。EDC HCl的潜在机制涉及它与羧基反应所形成的不稳定中间体，这种中间体会主动吸引胺伴侣（amine partner）。该反应的微妙平衡强调了我们需要优化条件以确保高效偶联。通过稳定中间体并实现两步偶联过程，N-羟基琥珀酰亚胺（NHS）的协助进一步增强了EDC HCl的能力，从而带来更大的灵活性和控制力，特别是在处理复杂生物分子的场合。

应用

  • N-(3-二甲基氨基丙基)-N′-乙基碳二亚胺盐酸盐已用于荧光纳米钻石(FND)-转铁蛋白生物偶联物的形成。
  • 它已用于聚乙烯亚胺与金颗粒的交联。
  • 它已用作碳二亚胺键合剂，用于将生物素化的BSA（牛血清白蛋白）涂覆在羧化的聚苯乙烯微珠上。

生化/生理作用

水溶性缩合试剂。EDAC通常用作与伯胺进行酰胺键合的羧基活化剂。另外，它能与磷酸基团反应。其已用于例如肽合成、将蛋白质与核酸交联、以及免疫缀合物的制备。通常，EDAC在不含缓冲剂的pH范围4.0-6.0的条件下使用。特别是，应避免使用胺和羧酸盐缓冲液。

特点和优势

用途广泛，适用于多种实验室和研究应用。

其他说明

For additional information on our range of Biochemicals, please complete this form.

可比产品

产品编号
说明
价格

相关产品

产品编号
说明
价格

通常也和此产品一起购买

产品编号
说明
价格

警示用语：

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

靶器官

Stomach,large intestine,lymph node

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Product No. E7750, N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, is listed as "commercial grade". What does that mean?

    It means that it is the grade of N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide (EDAC) hydrochloride that was commercially available at the time we purchased it. This product is not assayed for purity. If you need a high purity EDAC, consider Product No. 03450.

  4. I understand that E7750, N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, can be used in peptide synthesis. Do you have a brochure on some other reagents that can be used for peptide synthesis?

    Yes.

  5. Can N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, Product E7750, be used as a peptide coupling reagent in alcoholic solvents? If so, do you have a reference?

    Yes. Nosaki, S., Effects of amounts of additives on peptide coupling mediated by a water-soluble carbodiimide in alcohols. J. Peptide Res., 54(2), 162-167 (1999). This paper describes using the E7750 along with 3,4-dihydro-3-hydroxy-4-oxo-1,2,3-benzotriazine (HOOBt) and 1-hydroxy-7-azabenzotriazole (HOAt) for enhancement of peptide coupling.

  6. What impurities might be present in N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride, Product E7750?

    The two compounds most likely to appear in EDAC as trace impurities are 3-dimethylaminopropylamine and N-(3-dimethylaminopropyl)-N'-ethylurea as a result of synthesis (intermediate) and degradation (moisture or coupling reaction), respectively.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Fluorescent nanodiamonds for specifically targeted bioimaging: Application to the interaction of transferrin with transferrin receptor.
Weng M-F et al.
Diamond and Related Materials, 18, 587-587 (2009)
Mechanical checkpoint for persistent cell polarization in adhesion-naive fibroblasts.
Bun P et al.
Biophysical Journal, 107, 324-324 (2014)
Cationic gold microparticles for biolistic delivery of nucleic acids.
Svarovsky S et al.
Biotechniques, 45, 535-535 (2008)
Shengbiao Li et al.
Hepatology (Baltimore, Md.), 61(5), 1730-1739 (2015-01-22)
Valproic acid (VPA) is widely used to treat epilepsy, migraine, chronic headache, bipolar disorder, and as adjuvant chemotherapy, but potentially causes idiosyncratic liver injury. Alpers-Huttenlocher syndrome (AHS), a neurometabolic disorder caused by mutations in mitochondrial DNA polymerase gamma (POLG), is
Lars Freudenmacher et al.
The Journal of comparative neurology, 528(5), 705-728 (2019-10-01)
Based on anatomical and functional data, the habenula-a phylogenetically old brain structure present in all vertebrates-takes part in the integration of limbic, sensory, and basal ganglia information to guide effective response strategies appropriate to environmental conditions. In the present study
查看所有相关文献

商品

Introduction of Terminal Azide and Alkyne Functionalities in Polymers

Click chemistry, and the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) in particular, is a powerful new synthetic tool in polymer chemistry and material science.

Graphene Field Effect Transistors for Biological and Chemical Sensors

相关内容

Materials for Medical Devices Manufacturing | Sigma-Aldrich

This capabilities video explores our Contract manufacturing (CMO) expertise including our global supply chain, ISO 13485 manufacturing sites, quality systems and regulatory guidance for clinical device and IVD production.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门