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Merck
CN

69964

MRS 琼脂

NutriSelect® Basic, powder, nonselective for Lactobacillus Spp., pack of 500 g

别名:

按照 DeMan、Rogosa 和 Sharpe 方法的乳酸菌琼脂。

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UNSPSC代码:
41171605
NACRES:
NA.74

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无菌性

non-sterile

质量水平

100

表单

powder

保质期

limited shelf life, expiry date on the label

组成

agar, 12 g/L
dipotassium hydrogen phosphate, 2 g/L
D(+)-glucose, 20 g/L
magnesium sulfate (equivalent to 0.2 g/lheptahydrate), 0.1 g/L
manganous sulfate monhydrate, 0.05 g/L
meat extract, 5 g/L
sodium acetate, 5 g/L
triammonium citrate, 2 g/L
universal peptone, 10 g/L
yeast extract, 5 g/L

包装

pack of 500 g

制造商/商品名称

NutriSelect® Basic

技术

microbiological culture: suitable

最终pH

6.5±0.2 (25 °C)

应用

agriculture
bioburden testing
environmental
food and beverages
microbiology

适用性

nonselective for Lactobacillus Spp.
selective by low pH for Bifidobacterium spp.

相关类别

一般描述

MRS Agar, also known as DeMan-Rogosa-Sharpe Agar, is a microbiological medium specifically designed for the enrichment, cultivation, and isolation of Lactobacillus species from various materials. It is a non-selective medium with a low pH that favors the growth of Lactobacillus spp. and Bifidobacterium spp. The medium contains essential nutrients such as glucose, meat extract, yeast extract, and peptones, which provide nitrogenous and carbonaceous compounds, amino acids, and vitamins necessary for microbial growth. Sodium acetate and ammonium citrate enhance selectivity by inhibiting the growth of unwanted organisms. The media contains polysorbate (Tween 80), acetate, magnesium, and manganese, which are known to act as specific growth factors for Lactobacilli, and provide a rich nutrient base.

应用

用于从所有类型的材料中富集、培养、分离所有种类的乳杆菌。

特点和优势

NutriSelect® Basic is a powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

制备说明

取61.15g混悬于含有1 ml聚山梨醇80(TWEEN® 80; Sigma P8074)的1000 ml蒸馏水中。 煮沸以完全溶解培养基并充分混匀。15 lbs压力(121°C)高压蒸汽灭菌15分钟。如有需要，灭菌后用冰醋酸调节pH。

其他说明

我们现提供两种类型的培养基：GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基，满足用户不同层次的需求。
商标后的品级称号basic（基础）、plus（加强）或prime（特级）代表产品的不同质控水平，从基础的质控到标准质控加强版到全面合规的特级质控水平。

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物来源培养基
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN8759
BCCN3771
BCCN2036
BCCN1663
BCCM7898

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Hanan R Shehata et al.
Frontiers in microbiology, 11, 1095-1095 (2020-06-26)
Probiotics are defined as "live microorganisms that, when administered in adequate amounts, confer a health benefit on the host." The diverse health benefits have contributed to rapid increase in probiotic consumption and in the value of probiotic market, valued at
J.D. DeMan et al.
The Journal of Applied Bacteriology, 23, 130-130 (1960)
Rupal Mistry et al.
Genetics, 206(2), 889-904 (2017-04-18)
Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism's life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes
Eleftheria Barouni et al.
Food chemistry, 200, 76-82 (2016-02-03)
The object of the current study was to develop a new process for continuous Feta-type cheese production using a biocatalyst consisting of immobilized rennin on a tubular cellulose/starch gel (TC/SG) composite, which has been proven to be an appropriate carrier
Marie-Paule Nawrot-Esposito et al.
Insects, 11(10) (2020-10-18)
Bioinsecticides made from the bacterium Bacillus thuringiensis (Bt) are the bestselling bioinsecticide worldwide. Among Bt bioinsecticides, those based on the strain Bt subsp. kurstaki (Btk) are widely used in farming to specifically control pest lepidopteran larvae. Although there is much

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