Microbiological Media and Detection
Chloe McClanahan
HybriScan™ Detection
Molecular biology-based methods, like PCR, can be used for lactobacilli detection. However, they are often quite expensive. We provide a revolutionary molecular biology method that is rapid, easy, and cost effective. Based on the detection of rRNA, this method completely avoids the need for PCR amplification. The sandwich hybridization test, called HybriScan, is performed on a microtiter plate. The range of lactobacilli detected by HybriScan™ tests is listed in the table.
| Name | Application | Reactions | Cat. No. |
|---|---|---|---|
| HybriScan™ D Beer | Detection of all relevant beer spoilage organisms of genus Lactobacillus, Pediococcus, Pectinatus and Megasphaera | 96 reactions | 62533 |
| HybriScan™ D Drinks | Detection of bacteria and yeasts in fruit juices and nonalcoholic beverage; determination of total viable count | 96 reactions | 68301 |
| HybriScan™ D Lactobac | Detection of Lactobacilli in fruit juices and non-alcoholic beverage. | 96 reactions | 59744 |
| HybriScan™ I Lactobacillus brevis | Identification of Lactobacillus brevis | 48 reactions | 75724 |
| HybriScan™ I Lactobacillus buchneri | Identification of Lactobacillus buchneri | 48 reactions | 80065 |
| HybriScan™ I Lactobacillus lindneri | Identification of Lactobacillus lindneri | 48 reactions | 86827 |
Selective Media for Bifidobacteria
| Name | Application | Cat. No. |
|---|---|---|
| BSM Agar | For the selective isolation and identification of Bifidobacteria. The medium is used for quality control in the manufacture of dairy products. | 88517 |
| BSM Broth | For the selective isolation and identification of Bifidobacteria. The medium is used for quality control in the manufacture of dairy products. | 90273 |
| BSM Supplement | An antimicrobial supplement recommended for the selective isolation of Bifidobacteria. | 83055 |
| Wilkins Chalgren anaerobic agar | Used for the isolation of anaerobic bacteria. | W1761 |
Differentiation Media for Lactobacilli
| Name | Application | Cat. No. |
|---|---|---|
| Litmus Milk | For maintenance of Lactobacilli and for determining the action of bacteria on milk. | 17158 |
| LS Diff erential Agar | For the maximum growth and differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, 2,3,5-triphenyltetrazolium chloride reduction, and casein reaction. | 17153 |
| WL Nutrient Agar | For the examination of materials encountered in brewing and for industrial fermentations containing mixed fl ora of yeasts and bacteria. | 17222 |
| WL nutrient broth | Recommended for the cultivation of bacteria encountered in breweries and industrial fermentations. | W2261 |
Nonselective Media for Lactobacilli
| Name | Application | Cat. No. |
|---|---|---|
| Elliker Broth | For culturing Streptococci and Lactobacilli of importance in the dairy industry. | 17123 |
| Plate Count Skim Milk Agar | For the enumeration of bacteria in milk and dairy products. | 80957 |
| Tomato Juice Agar | For the cultivation and enumeration of Lactobacilli. | 17216 |
| Tomato Juice Broth | For cultivation of yeasts and other aciduric microorganisms. | 17218 |
| Tryptone Glucose Yeast Extract Agar | Recommended for enumeration of bacteria in water, air, milk and dairy products. | T2188 |
| Yeast malt agar | Used for the isolation and cultivation of yeasts, molds and other aciduric microorganisms. | Y3127 |
Selective Media (by low pH and selective agents) for Lactobacilli
| Name | Application | Cat. No. |
|---|---|---|
| Lactobacillus bulgaricus Agar (Base) | Used with acetate buffer for isolation and identification of Lactobacillus bulgaricus. | 17154 |
| MRS Agar | For the enrichment, cultivation, and isolation of all species of Lactobacillus from all types of material according to DeMan, Rogosa, and Sharpe. | 69964 |
| MRS Agar, original acc. DeMan-Rogosa-Sharpe | For the enrichment, cultivation, and isolation of all species of Lactobacillus from all types of materials. Recommended by the "Schweizerisches Lebensmittelbuch" 5th ed., chapter 56A. | 30912 |
| MRS Agar, Vegitone | This MRS Agar is free of animal-derived material. It is recommended for the isolation and cultivation of Lactobacillus species. | 41782 |
| MRS Broth | For the enrichment and isolation of all species of Lactobacilli from all types of material. | 69966 |
| MRS Broth modifi ed, Vegitone | This MRS Broth contains plant peptone instead of animal peptone. It is recommended for the isolation and cultivation of Lactobacillus species. | 38944 |
| NBB Agar Base, modified | Selective medium used for the detection of contaminating/spoilage microorganisms in brewery. | 64198 |
| NBB Broth Base, modified | Selective medium used for the detection of contaminating/spoilage microorganisms in brewery. | 50725 |
| Orange-serum Agar | For the isolation, cultivation and enumeration of acid-tolerant spoilage microorganisms in fruit juice and fruit juice concentrates, in particular from citrus fruit, according to Hays, Troy, and Beisel. The prepared agar may be turbid after autoclaving due to orange extract. | 75405 |
| Raka-Ray Agar | A medium for selective isolation of lactic acid bacteria from beer and brewing processes | 02538 |
| Rogosa SL agar | Used as a selective medium for cultivation of Lactobacilli. | R1148 |
| Sorbic acid Agar (Base) | For the isolation and differentiation of Lactobacilli from food, feces, etc. according to Reuter. | 85515 |
| Universal Beer Agar | For culturing microorganisms of significance in the brewing industry. | 17226 |
| WL Diff erential Agar | For selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations. | 17215 |
Additional Materials
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