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Merck
CN
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文件

安全信息

17222

Millipore

WL 营养琼脂

suitable for microbiology, NutriSelect® Plus,

for cultivation of microorganisms encountered in the brewing and fermentation industries

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别名:
Wallerstein 实验室营养琼脂
UNSPSC代码:
41171605
NACRES:
NA.74

质量水平

200

无菌性

non-sterile

形式

powder

保质期

limited shelf life, expiry date on the label

组成

agar, 20 g/L
bromocresol green, 0.022 g/L
calcium chloride, 0.125 g/L
casein enzymic hydrolysate, 5 g/L
dextrose, 50 g/L
ferric chloride, 0.0025 g/L
magnesium sulfate, 0.125 g/L
manganese sulfate, 0.0025 g/L
monopotassium phosphate, 0.55 g/L
potassium chloride, 0.425 g/L
yeast extract, 4 g/L

制造商/商品名称

NutriSelect® Plus

技术

microbiological culture: suitable

最终pH

5.5±0.2 (25 °C)

应用

bioburden testing
food and beverages
microbiology

适用性

nonselective and differential for Candida spp.
nonselective and differential for Escherichia coli
nonselective and differential for Lactobacillus spp.
nonselective and differential for Pichia spp.
nonselective and differential for Proteus spp.
nonselective and differential for Saccharomyces spp.
nonselective and differential for Zygosaccharomyces spp.
nonselective and differential for bacteria (General Media)
nonselective and differential for coliforms
nonselective and differential for molds (General Media)
nonselective and differential for yeasts (General Media)

相关类别

一般描述

WL（Walerstein Laboratory）培养基遵照Green和Gray的配方配制，可用于检测酿造过程中的材料以及含有酵母和细菌混合菌群的工业发酵物。酵母提取物是维生素、氨基酸和痕量元素的来源。酶水解酪蛋白提供氮、氨基酸和碳。右旋糖是主要的碳和能量来源。磷酸钾是一种缓冲剂。培养基中的多种盐提供必需的离子，并有助于维持渗透平衡。溴甲酚绿作为pH指示剂，而琼脂则作为固化剂。WL营养琼脂是用于检测酿造过程中的材料以及含有酵母和细菌混合菌群的工业发酵物。

应用

WL营养琼脂适用于：
  • 使酵母母种复活并用于发酵后的种群动力学研究
  • 从奶酪中分离的酵母的形态表征
  • 用于平板培养发酵的葡萄酒的酵母菌分离

制备说明

将80.25 g悬浮于1L蒸馏水中。加热至沸腾以完全溶解培养基。在121°C加热15分钟进行高压蒸汽灭菌。如果需要得到6.5的pH值，添加1%碳酸氢钠溶液。

附注

我们现提供两种类型的培养基：GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基，满足用户不同层次的需求。
商标后的品级称号basic（基础）、plus（加强）或prime（特级）代表产品的不同质控水平，从基础的质控到标准质控加强版到全面合规的特级质控水平。

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物来源培养基

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Paper read at American Society of Brewing Chemists Meeting
Green, SR and Gray, PP
Wallerstein Laboratories Communications, 12, 43-43 (1950)
Selection of autochthonous Saccharomyces cerevisiae strains for production of typical Pinot Gris wines.
Schvarczova E, et al.
Journal of Food & Nutrition Research, 56(4) (2017)
The impact of Saccharomyces cerevisiae on a wine yeast consortium in natural and inoculated fermentations
Bagheri B, et al.
Frontiers in Microbiology, 8, 1988-1988 (2017)
The rapid detection of brewery spoilage micro-organisms
Harrison J, et al.
Journal of the Institute of Brewing, 80(4), 390-398 (1974)
Characterisation of yeast flora isolated from an artisanal Portuguese ewe's cheese
Malfeito-Ferreira M, et al.
International Journal of Food Microbiology (2000)

商品

Culture Method for Beer Quality Control

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Microbiological Media & Detection

Cost-effective molecular biology method for lactobacilli detection is presented as an alternative to PCR-based methods.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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