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SITRAN-RO

Roche

X-tremeGENE^™ siRNA 转染试剂

Name: X-tremeGENE<SUP>™</SUP> siRNA 转染试剂
Brand: ROCHE

Polymer reagent for delivering siRNA to common cell lines

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About This Item

UNSPSC代码:

41106502

NACRES:

NA.55

等级

for molecular biology

质量水平

100

用途

sufficient for 2,000 transfections (04476115001)
sufficient for 400 transfections (04476093001)

包装

pkg of 1 mL (04476093001 [1 mg/ml])
pkg of 5 × 1 mL (04476115001 [5 x 1 mg/ml])

制造商/商品名称

Roche

浓度

1 mg/mL

技术

transfection: suitable

运输

wet ice

储存温度

2-8°C

一般描述

转染操作可将小干扰RNA（siRNA）直接递送至细胞中。X-tremeGENE siRNA转染试剂是一种脂质体转染试剂，为向动物细胞中递送siRNA提供了一种方便、可靠和高效的途径，便于用户进行基因敲除后的细胞及功能学研究。
这一创新型试剂能够与siRNA以及siRNA和质粒的混合物（共转染的情况下）形成复合物，并有效递送这些核酸进入细胞，以介导基因沉默。只需简单的几步就可完成转染：首先将转染试剂与稀释过的siRNA混合并孵育，之后将复合物直接加入细胞培养物中。由于X-tremeGENE siRNA转染试剂在有无血清的条件下均工作良好，且经论证无明显细胞毒性，因此无需更换培养基。本品不含任何动物源性成份。

组分

本试剂经0.2 μm滤膜过滤，聚丙烯小管包装。

应用

X-tremeGENE siRNA 转染试剂能够有效将短干扰RNA（siRNAs）转入许多种常用细胞，包括HeLa，NIH 3T3，HEK-293，CHO-K1，和COS-7和一些难转染细胞系，比如HT29，一种人腺癌细胞系。

特点和优势

在多种不同类型细胞中基因敲除效率超过90%。
高灵活应用，同一试剂可用于siRNA和质粒共转染的基因沉默实验。
细胞毒性低，结果相关性好，确保所观察到的细胞效应是由转染的siRNA而非转染过程本身介导。
兼容含血清或不含血清的培养基，转染前后均无需换液（如无血清培养基）。;

X-tremeGENE siRNA转染试剂是一款包含脂质体及其他组份的专利混合试剂，无动物源成分。

质量

活性分析：将X-tremeGENE siRNA转染试剂（1 - 2.5 μl）与特异性靶向HPRT看家基因的siRNA（0.1 - 0.35 μg）相互混合。在含10%胎牛血清（FBS）的条件下，将此混合物用于转染HEK-293细胞（生长密度为30-50%的单层细胞）。转染后通过LightCycler®实时荧光定量PCR来检测细胞中HPRT mRNA水平的降低情况。测得的mRNA数据显示，观察到的敲除效率通常处于70-95%。

细胞毒性分析：HEK-293细胞暴露于siRNA/X-tremeGENE siRNA试剂混合物中72小时，未对含有血清的培养基进行换液。之后通过WST-1细胞增殖试剂（Roche）分析细胞，以测试细胞毒性。

其他说明

仅用于生命科学研究。不可用于诊断。

法律信息

X-tremeGENE is a trademark of Roche

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

does not flash

闪点(°C)

does not flash

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Scintillation proximity assay for measuring uptake by the human drug transporters hOCT1, hOAT3, and hOATP1B1.

Christina Lohmann et al.

Analytical biochemistry, 366(2), 117-125 (2007-06-09)

Increasing evidence suggests a key role of transport proteins in the pharmacokinetics of drugs. Within the solute carrier (SLC) family, various organic cation transporters (OCTs), organic anion transporters (OATs), and organic anion transporting polypeptides (OATPs) that interact with drug molecules

Influences on hepatitis B virus replication by a naturally occurring mutation in the core gene.

Masaya Sugiyama et al.

Virology, 365(2), 285-291 (2007-05-15)

Little is known about specific naturally occurring mutations of hepatitis B virus (HBV) and underlying mechanisms of their association with fulminant hepatitis. A HBV clone isolated from a patient with fulminant hepatitis was analyzed, and the features of the particular

MicroRNA expression dynamics during murine and human erythroid differentiation.

Mei Zhan et al.

Experimental hematology, 35(7), 1015-1025 (2007-06-26)

MicroRNAs (miRNAs) are an abundant class of small noncoding RNAs that regulate diverse cellular functions by sequence-specific inhibition of gene expression. We determined miRNA expression profile during erythroid differentiation and putative roles in erythroid differentiation. The expression profile of 295

Adenylyl cyclase 6 deletion reduces left ventricular hypertrophy, dilation, dysfunction, and fibrosis in pressure-overloaded female mice.

Tong Tang et al.

Journal of the American College of Cardiology, 55(14), 1476-1486 (2010-04-03)

This study sought to test the hypothesis that pressure stress of the adenylyl cyclase 6-deleted (AC6-KO) heart would result in excessive hypertrophy, early dilation and dysfunction, and increased fibrosis. Cardiac-directed AC6 expression attenuates left ventricular (LV) hypertrophy and dysfunction in

Suppression of Nrf2-driven heme oxygenase-1 enhances the chemosensitivity of lung cancer A549 cells toward cisplatin.

Hak-Ryul Kim et al.

Lung cancer (Amsterdam, Netherlands), 60(1), 47-56 (2007-11-17)

Heme oxygenase-1 (HO-1) is highly expressed in various tumor tissues and plays an important role in tumor cell growth through anti-oxidative and anti-apoptotic effects. Herein, we demonstrate that A549 cells express high levels of HO-1, Nrf2, and NF-kappaB compared to

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商品

X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs offer easy gene expression knockdown in mammalian cells, revolutionizing gene research.

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

细胞转染简介

转染是将DNA、RNA或蛋白质引入真核细胞的过程，用于研究和调节基因表达。因此，转染技术和实验方案作为分析工具，有助于表征遗传功能、蛋白质合成、细胞生长和发育。

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

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实验方案

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

利用X-tremeGENE™ 转染试剂转染常见细胞系的实验方案

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

文件

X-tremeGENE™ siRNA 转染试剂

Polymer reagent for delivering siRNA to common cell lines

About This Item

属性

等级

质量水平

用途

包装

制造商/商品名称

浓度

技术

运输

储存温度

相关类别

说明

一般描述

应用

特点和优势

质量

其他说明

法律信息

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储存分类代码

WGK

闪点(°F)

闪点(°C)

文件

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X-tremeGENE^™ siRNA 转染试剂