跳转至内容
Merck
CN
所有图片(1)

文件

安全信息

D5796

Sigma-Aldrich

DMEM - high glucose

With sodium Bicarbonate and ʟ-glutamine, without sodium pyruvate, sterile liquid, suitable for cell culture

登录查看公司和协议定价
所有图片(1)
别名:
DME, DMEM
UNSPSC代码:
12352207
NACRES:
NA.75

product name

杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

质量水平

400

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no

运输

ambient

储存温度

2-8°C

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

这种DMEM高葡萄糖培养基是一种不含添加因子（常见修改）如L-丙氨酰-L-谷氨酰胺、HEPES或丙酮酸钠的1x完全培养基。 它与原始DMEM高葡萄糖配方的不同点在于用吡哆醇代替了吡哆醛。 吡哆醛是一种不稳定的培养基成分。

应用

Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。

推荐

产品编号
说明
价格

相关产品

产品编号
说明
价格

补充剂

产品编号
说明
价格

通常也和此产品一起购买

产品编号
说明
价格

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Christian Kirschneck et al.
Scientific reports, 7(1), 14751-14751 (2017-11-09)
Meaningful, reliable and valid mRNA expression analyses by real-time quantitative PCR (RT-qPCR) can only be achieved, if suitable reference genes are chosen for normalization and if appropriate RT-qPCR quality standards are met. Human periodontal ligament (hPDL) fibroblasts play a major
Refael Minnes et al.
Scientific reports, 7(1), 4381-4381 (2017-07-01)
The vast majority of cancer related deaths are caused by metastatic tumors. Therefore, identifying the metastatic potential of cancer cells is of great importance both for prognosis and for determining the correct treatment. Infrared (IR) spectroscopy of biological cells is
Saabah B Mahbub et al.
Scientific reports, 7(1), 15792-15792 (2017-11-19)
Bioimaging using endogenous cell fluorescence, without any external biomarkers makes it possible to explore cells and tissues in their original native state, also in vivo. In order to be informative, this label-free method requires careful multispectral or hyperspectral recording of
Tomoyuki Muroi et al.
Reproduction (Cambridge, England), 154(6), 755-764 (2017-09-16)
G-protein-coupled receptors (GPCRs) participate in diverse physiological functions and are promising targets for drug discovery. However, there are still over 140 orphan GPCRs whose functions remain to be elucidated.
Kai-Liang Zhang et al.
OncoTargets and therapy, 12, 6515-6523 (2019-10-17)
The efficacy of traditional therapies for oral carcinoma (OC) is limited. Oncolytic adenovirus, a novel strategy of cancer therapy, shows potential use in OC treatment. However, its clinical application is limited by pre-existing neutralizing antibodies. Thus, this study aimed to
查看所有相关文献

商品

3D类器官培养：用于开发及疾病研究的新体外模型

可生成组织和干细胞来源3D脑、胃肠道、肺和癌症肿瘤类器官模型的类器官培养产品。

3D Organoid Culture: New In Vitro Models of Development and Disease

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

实验方案

Primary Human Hepatic Sinusoidal Endothelial Cells Culture Protocol

Discover our collection of primary human hepatic sinusoidal endothelial cells, a protocol for thawing, plating, and growing HHSECs, and a recipe for our HHSEC maintenance media formulation.

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

慢病毒转导实验方案

如何利用MISSION shRNA慢病毒颗粒进行慢病毒转导，以实现稳定的长期沉默和表型变化的详细操作步骤。

相关内容

Primary Human Hepatic Stellate Cells Culture Protocol

Discover our collection of primary human hepatic stellate cells and protocol for thawing, plating, and growing stellate cells. Find our stellate culture maintenance media formulation.

Cell Culture Media Preparation Using MilliShot™ Single Dose Antibiotics

Learn how to use our cell culture tested, ready-to-use MilliShot™ single dose antibiotic solutions, conveniently packaged in one-time use vials.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门