CRISPR和基因编辑
CRISPR CAS9蛋白必备品和服务的终极套装
随着基因组工程技术的进步，从治疗遗传疾病到开发抗病作物，研究者在各个领域都取得前所未有的突破。我们可靠的CRISPR Cas9蛋白必备品和工具全面支持从实验室到大规模的每一步CRISPR基因编辑研究。提供可靶向单个基因的锌指核酸酶或用于筛选全基因组可全面定制的CRISPR产品。
创新来自日复一日的工作积累，只有仔细选择CRISPR Cas9蛋白和基因编辑工具，打下良好基础，才不会因劣质品而前功尽废。
概要
Sigma-Aldrich® Advanced Genomics产品线拥有基因组工程工具和服务的完整套件，可全方位释放您的研究潜能。无论您是要敲除、敲入、敲低，还是要过表达靶基因，我们全套的CRISPR基因编辑工具和服务都将满足您实验台之外的各种研究需求。
同类最佳的Cas9蛋白
Sigma-Aldrich® Advanced Genomics提供各种Cas9突变体(野生型、特异性增强型、nickase切口酶型、GFP融合型、催化失活型)，形式选择多样（如质粒、慢病毒、冻干蛋白粉）。探索我们行业领先的Cas9蛋白系列，找到满足您的不同研究需求的产品。
- 查看我们Cas9产品的完整列表
- 了解更多有关Cas9-GFP融合蛋白的信息
兼容10x Genomics的CRISPR筛选
Sigma-Aldrich® Advanced Genomics拥有兼容10x Genomics认证的慢病毒库，可用于CRISPR单细胞筛选。我们优化定制的慢病毒载体与采用Feature Barcode技术的Chromium单细胞基因表达v3和v3.1 (Next GEM)解决方案完全兼容。
- 了解更多有关兼容10x Genomics产品的信息
- 双gRNA 载体让您的编辑能力翻番。
我们可随时为您提供帮助。
无论您是要进行CRISPR筛选实验设计，还是要排查故障，或只是想更好地认识如何在研究中运用基因编辑方法，我们的Sigma-Aldrich® Advanced Genomics团队都可随时为您提供指导帮助。
担心CRISPR不适合您的实验项目？连线咨询Sigma-Aldrich®Advanced Genomics专家。
相关产品资源
- Article: Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli
In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template.
- CRISPR使用许可协议
This Product and its use are the subject of one or more of the following patents controlled by The Broad Institute, Inc. (BROAD), the Massachusetts Institute of Technology (MIT), or the President and Fellows of Harvard College (HARVARD): U.S. Patent Nos. 8,697,357; 8,771,945, and any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions, and corresponding foreign patent applications and patents.
- Article: Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
- Article: Genome Editing in Plants with CRISPR/Cas9
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field, opening up new possibilities for research and development.
- eBook: Gene Editing 101
Learn how to understand, design and perform CRISPR experiments, as well as design gRNA, choose a Cas9 format, screen with CRISPR, use advanced CRISPR formats, and much more…
- Article: CRISPR Cas9 Protein Product Selection Guide
A convenient CRISPR Cas9 protein selection guide to assist with your CRISPR RNP, lentivirus, mRNA, plasmids, vectors and control needs.
- Article: Blue-White Screening & Protocols for Colony Selection
Our popular blue-white screening for colony selection technical resource hub, transfection reagent selection guide and protocols.
- PCR Master Mix Calculator
Ensure the success of your reactions at any scale with our handy PCR Master Mix Calculator resource tool.
- Article: Introduction to Cell Transfection
An overview on cell transfection vs transduction methods, our cell transfection reagent selection guide and protocols.
- Protocol: Western Blotting
Western blotting protocol that includes technical information supporting the following: Sample preparation, membrane transfer/tank transfer, transfer stack assembly, buffers, immunodetection, and a troubleshooting guide.
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