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PCR用品和仪器

聚合酶链式反应(PCR)是强大的分子生物学核心技术,可从各种来源核酸中快速有效地体外酶促扩增特定DNA和RNA序列。简单的PCR反应由合成寡核苷酸引物(与目标DNA序列侧翼结合)、目标DNA、一种热稳定DNA聚合酶和dNTP组成。重复循环过程包括模板变性、引物退火及退火引物的延伸,最终产生大量DNA。而循环中新合成的每条DNA链都会成为下一个循环中的模版,只需20个循环DNA就可扩增至100万倍。从PCR微孔板、板盖、移液器吸头、PCR管到热循环仪,探索我们全面的PCR耗材和仪器,支持您PCR流程的每一步骤。 

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用于DNA扩增的PCR 热循环仪和微型循环仪

热循环仪(也称为PCR仪)是一种在重复循环中加热和冷却样品,以便于聚合酶链式反应进行DNA和RNA扩增的实验室仪器。这类仪器已惯常占据实验室一角,但随着微型或便携式热循环仪的引入,在现场和教室进行PCR反应已变得更加方便。很多热循环仪都是可编程的,用户可以保存常用程序。PCR通常需要20至35个循环,每个循环由2至3个温度步骤组成(变性、退火和延伸)。Eppendorf® Mastercycler热循环仪兼具速度、灵活性和PCR优化优势,可提供2D梯度和全面记录能力等PCR增强功能。此外,我们还提供Benchmark Scientific TC型热循环仪,其设计用于满足较低通量需求,与大型装置相比具有相同的功能且价格较低。 

此外,请浏览各种其他PCR板密封件,包括eXTReme™板密封件、ThermalSeal RTS™板密封件和AlumaSeal®PCR板密封件,以满足您的PCR工作流程需求。

PCR板、条和膜

BRAND® PCR产品可保护您的样品免遭污染和蒸发。洁净室生产、高品质原料及由认证实验室执行的完善的质量控制保证了稳定的高标准洁净度。适配PCR板、管的封板膜和盖子极大减少了蒸发损失,微量样品也能可靠分析。BRAND® PCR耗材都不含DNase、DNA、RNase和内毒素。我们还提供结合了聚丙烯和聚碳酸酯材料优点的Eppendorf®twin-tec PCR板,而Corning®Thermowell PCR 96孔板适用于大多数热循环仪,是高通量自动化的理想选择。

PCR移液器吸头和滤芯吸头

在我们的产品选择表中查看各种可靠的移液器吸头,包括市场领先的ART® 移液器吸头。此外,BRAND®移液器吸头和滤芯吸头在最现代化的生产条件下在洁净室中制造,并自动进行吸头架装配和包装,以确保吸头始终保持高品质。BRAND®移液器吸头和滤芯吸头用高纯度聚丙烯制造,不含DiHEMDA、油酰胺、DNA(<40fg)、RNase(<8.6fg)、内毒素(<1 pg)和ATP(<1fg)。另外,我们荣幸地提供一系列Eppendorf®产品,包括用于液体处理的Eppendorf移液器吸头,适合快速处理并避免交叉污染。我们根据容量、无菌和非无菌以及吸头盒要求,针对细分应用提供了各种Eppendorf®移液吸头。另外,Axygen耗材除了移液吸头外还有各种微孔板以满足您的特别应用需要。 


相关产品资源

  • Brochure: Reagents for PCR

    Roche has provided the gold standard in PCR reagents. Our advanced isolation, purification, and manufacturing techniques ensure that you receive not only the clearest, most reliable results, but also convenience and affordability to fit any laboratory budget.

  • Brochure: PCR

    Suitable for use with 96-well PCR plates as well as standard and low profile Thermo-Strips. Easy to apply without the aid of a capping tool. The flat surface of each cap facilitates sample labeling using a marker pen or adhesive stickers and large labeled end-tabs aid orientation and removal.

  • Tech Article: Complete Solutions for PCR Assay Development

    We offer a wide variety of Polymerase Chain Reaction (PCR) reagents, kits and tools to meet your experimental needs at any stage of development or manufacturing.

  • Protocol: PCR/qPCR/dPCR Assay Design

    The process of assay design is greatly facilitated by adoption of suitable design software. OligoArchitect provides two options for design support. The first is OligoArchitect Online, a software design tool with a wide range of options. If the design requires a specialized capability, the second option is to request the design via OligoArchitect Consultative, utilizing the assistance of our expert molecular biologists.

  • Protocol: FFPE Tissue

    Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases. Since the archived DNA is usually available in limited quantities, amplification of the samples is essential.

  • Quick Reference Guide: ProbeFinder
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