MAB2166
Anti-Huntingtin Disease (HD/HTT) Antibody
CHEMICON®, mouse monoclonal, 1HU-4C8
别名:
Huntingtin, Huntington′s Disease Protein, HD Protein
产品名称
抗亨廷顿蛋白抗体，a.a.181-810，克隆 1HU-4C8, ascites fluid, clone 1HU-4C8, Chemicon®
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1HU-4C8, monoclonal
species reactivity
rat, rabbit
species reactivity (predicted by homology)
mouse, human, hamster, monkey
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HTT(3064), SLC6A4(6532)
Analysis Note
正常人大脑皮层裂解物
蛋白质印迹分析：
该批次以1：1000的稀释度在10 μg大鼠脑裂解物中检测到亨廷顿蛋白。
Application
先前批次的1:500-1:5,000的稀释液被用于ELISA。
免疫组化：
将先前批次的1：500-1：5,000稀释液用于冷冻和微波炉处理的石蜡切片（人体组织）。
免疫细胞化学：
先前批次的1：500-1：5,000用于转染细胞。
免疫沉淀：
先前批次的1:500-1:5,000的稀释液被用于免疫沉淀。
蛋白质印迹：
1:500-1:5,000。应通过蛋白质印迹检测到大约350-400 kDa处迁移的条带（Nature Genetics 10：104-110.）。
最佳工作稀释度必须由最终用户进行确定。
神经退行性疾病
神经科学
Biochem/physiol Actions
Disclaimer
General description
Immunogen
Other Notes
Physical form
Preparation Note
使用建议：收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于 -20°C。避免反复冻融循环，以免损坏 IgG 和影响产品性能。
Legal Information
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.
免疫荧光法(IF)通过在抗体标记上荧光分子，然后使用激光器激发荧光，包括蛋白质的定位、验证翻译后修饰或活化以及与其他蛋白质的邻近和复合等操作。
实验方案
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
相关内容
Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.
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