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主要文件

AP112P

Sigma-Aldrich

山羊抗人IgG抗体,HRP偶联

Chemicon®, from goat

别名:

Anti-Human IgG HRP Conjugate

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2 ML
CN¥3,422.53

CN¥3,422.53


预计发货时间May 06, 2025详情


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2 ML
CN¥3,422.53

About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.46

CN¥3,422.53


预计发货时间May 06, 2025详情


获取大包装报价

生物来源

goat

质量水平

偶联物

peroxidase conjugate

抗体形式

affinity isolated antibody

抗体产品类型

secondary antibodies

克隆

polyclonal

表单

lyophilized

种属反应性

human

制造商/商品名称

Chemicon®

技术

ELISA: suitable
western blot: suitable

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此商品
AP309PAP307PAP114P
conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

species reactivity

human

species reactivity

human

species reactivity

rabbit

species reactivity

human

biological source

goat

biological source

goat

biological source

goat

biological source

goat

isotype

IgG

isotype

IgG

isotype

IgG

isotype

-

一般描述

免疫球蛋白G(IgG)属于免疫球蛋白家族,是一种广泛表达的血清抗体。这是一种糖蛋白,主要帮助免疫防御系统。大约70%的总免疫球蛋白由IgG组成。IgG是一种单体免疫球蛋白,由四条多肽链组成。它由两条50 kDa重链(γ)和两条25 kDa轻链组成。这些链通过二硫键相互连接。每个分子有两个抗原结合位点。IgG是最丰富的免疫球蛋白。它在血液和组织液中的分布大致相等,占人血清免疫球蛋白的75%。有4个子类:IgG1(66%),IgG2(23%),IgG3(7%)和IgG4(4%)。

特异性

该抗体与人IgG的重链和大多数人免疫球蛋白共有的轻链反应。

免疫原

由纯化人IgG制备。

应用

山羊抗人IgG抗体,HRP偶联物已用于:
  • 在酶联免疫吸附测定(ELISA)中检测结合的免疫球蛋白G(IgG)
  • 在免疫组化或细胞化学中,稀释度为1:500-1:5,000
  • 用于稀释度为1:5,000-1:100,000的显色底物和稀释度为的1:10,000-1:200,000的ECL底物的蛋白质印迹。
  • 在EIA中,稀释度为1:5,000-1:100,000

生化/生理作用

免疫球蛋白G(IgG)参与II型和III型超敏反应。它启动补体系统的经典途径,也中和病毒颗粒和毒素。母体IgG可通过人胎盘传递给胎儿,为新生儿抵抗感染提供免疫防御。它促进抗体依赖性细胞介导的细胞毒性(ADCC)。IgG被木瓜蛋白酶消化产生抗原结合片段(Fab)。IgG的胃蛋白酶消化导致可结晶片段(Fc)的形成。严重急性呼吸综合征冠状病毒(SARS-CoV)-2感染患者的IgG水平可能有助于评估2019冠状病毒病(COVID-19)的严重程度和预后。

外形

使用与琼脂糖珠偶联的抗原通过免疫亲和层析纯化。冻干。缓冲液为0.01 M磷酸钠,0.25 M NaCl,pH值7.6和15 mg/ml BSA。不含防腐剂。

储存及稳定性

在接收之日起,将冻干产品在产物2°-8°C下保存长达12个月。复溶后,产品作为未稀释的液体在2°-8°C下稳定6周。用2 mL蒸馏水复溶小瓶。要延长复溶后溶液的保存时间,请添加等体积的甘油,使甘油的最终浓度达到 50%,然后以未稀释的等分试样置于 -20°C 下可保存长达 12 个月。请注意,添加甘油后,抗体(和缓冲盐)的浓度将降低至原浓度的一半,并且必须相应地调整最终的工作稀释度。应避免反复冻融循环。

警告:
使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。

其他说明

研究子类别
完整免疫球蛋白二抗
研究类别:二级&对照抗体

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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