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产品名称
Chromolith® HighResolution RP-18 封端, L × I.D.150 mm x 4.6 mm HPLC column
物料
PEEK column
质量水平
Agency
suitable for USP L1
产品线
Chromolith®
特点
endcapped
endcapped
包装
1 ea of
标记范围
15% Carbon loading
18% Carbon loading
参数
200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.
技术
HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)
色谱柱长度 × 内径
15 cm × 4.6 mm
表面积
250 m2/g × 250 m2/g
基质
highly porous
monolithic silica
monolithic silica
基质活性基团
C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase
孔径
1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity
工作pH范围
2-7.5
2-7.5
分离技术
reversed phase
储存温度
no temp limit
一般描述
Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping.
Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column. Chromolith® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 150 mm length and 4.6 mm I.D. is the best choice for high-resolution separations of complex compound mixtures on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025]
with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Chromolith® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns
法律信息
储存分类代码
11 - Combustible Solids
商品
Validated HPLC method (based on Ph. Eur. Monograph 2668) for quantifying catechins and caffeine in decaffeinated green tea using a Chromolith® High Resolution RP-18e column with advantages in terms of backpressure and matrix robustness.
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
相关内容
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
小分子特指低分子量化合物（通常小于900道尔顿），分离小分子方法包括高效液相色谱(HPLC)、液相(LC)、气相(GC)、薄层色谱(TLC) 和毛细管电泳(CE)。此外，还可通过磁共振波谱法(NMR)或 质谱法(MS)等方法鉴定小分子。
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