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Agency
ISO 7899-2
质量水平
形式
medium granules (dehydrated (DCM))
包装
pkg of 500 g
制造商/商品名称
GranuCult® plus
储存条件
protect from light (Keep tightly closed and dry)
技术
microbe id | specific enzyme detection: suitable
microbiological culture: suitable
颜色
yellow
pH值（酸碱度）
6.9-7.3 (25 °C, 54.85 g/L in H2O, after autoclaving)
溶解性
54.85 g/L
应用
food and beverages
microbiology
储存温度
15-25°C
适用性
Enterococcus spp.
Streptococcus spp.
一般描述
GranuCult® plus Bile Esculin Azide Agar is a selective medium used for isolation and presumptive identification of fecal Streptococci. The presence of intestinal enterococci serves as an indicator of fecal contamination. BEA agar is employed as a confirmation and enumeration of medium for typical isolation on the primary isolation Membrane Enterococcus Selective Agar (1.05262 and 1.05289). Enterococci and some species of the genus Streptococcus namely S. bovis and S. equines can reproduce normally in this medium. Esculin hydrolysis and bile tolerances are regarded as reliable characteristics of enterococci. Intestinal enterococci hydrolyze the glycoside esculin to give dextrose and esculetin, which forms an olive green to black complex with iron (III) ions.Enterococci are bile tolerant. Bile salts exhibit the growth of numerous accompanying bacteria. The concentration of sodium azide in the medium inhibits the growth of Gram-negative microbial flora while sparing the enterococci.
应用
GranuCult® plus Bile Aesculin Azide Agar is suitable for the detection and enumeration of intestinal enterococci (fecal streptococci).
特点和优势
- GranuCult® offers superior granulated culture media
- Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
- Excellent wettability, solubility, and free-flowing properties
- Convenient, with minimal component separation and clumping, even under warm or humid conditions
- High batch-to-batch reproducibility
- Prolonged shelf life of up to five years
- High number of test strains exceeding all regulatory demands
- Granulation technology allows many supplements to be included, with no need to add these separately
分析说明
Appearance (clearness): clear
Appearance (colour): green-yellow irridescent
pH-value (25 °C): 6.9 - 7.3
Recovery on test medium (Enterococcus faecium ATCC 882): ≥ 60 %
Recovery on test medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 70 %
Recovery on test medium (Enterococcus durans ATCC 6056): ≥ 50 %
Recovery on test medium (Enterococcus hirae ATCC 8043 (WDCM 00089)): ≥ 60 %
Recovery on test medium (Listeria monocytogenes ATCC 19118): ≤ 0.01 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 0.01 %
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): fair
Colony colour (Enterococcus faecium ATCC 882): black
Colony colour (Enterococcus faecalis ATCC 19433 (WDCM 00009)): black
Colony colour (Enterococcus durans ATCC 6056): black
Colony colour (Enterococcus hirae ATCC 8043 (WDCM 00089)): black
Colony colour (Listeria monocytogenes ATCC 19118): colourless
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)): colourless
Colony colour (Staphylococcus aureus ATCC 25923 (WDCM 00034)): colourless
Incubation: 24 hrs.; 35°C; aerobic.
Appearance (colour): green-yellow irridescent
pH-value (25 °C): 6.9 - 7.3
Recovery on test medium (Enterococcus faecium ATCC 882): ≥ 60 %
Recovery on test medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 70 %
Recovery on test medium (Enterococcus durans ATCC 6056): ≥ 50 %
Recovery on test medium (Enterococcus hirae ATCC 8043 (WDCM 00089)): ≥ 60 %
Recovery on test medium (Listeria monocytogenes ATCC 19118): ≤ 0.01 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 0.01 %
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): fair
Colony colour (Enterococcus faecium ATCC 882): black
Colony colour (Enterococcus faecalis ATCC 19433 (WDCM 00009)): black
Colony colour (Enterococcus durans ATCC 6056): black
Colony colour (Enterococcus hirae ATCC 8043 (WDCM 00089)): black
Colony colour (Listeria monocytogenes ATCC 19118): colourless
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)): colourless
Colony colour (Staphylococcus aureus ATCC 25923 (WDCM 00034)): colourless
Incubation: 24 hrs.; 35°C; aerobic.
附注
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法律信息
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
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