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描述
0.2 μm sterile filtered
suitable for 3D bioprinting applications
表单
viscous liquid
杂质
≤5 CFU/g Bioburden (Fungal)
≤5 CFU/g Bioburden (Total Aerobic)
颜色
colorless to pale yellow
pH值（酸碱度）
6.5-7.5
应用
3D bioprinting
储存温度
2-8°C
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一般描述
3D bioprinting is the printing of biocompatible materials, cells, growth factors, and the other supporting materials necessary to yield functional complex living tissues. 3D bioprinting has been used to generate several different types of tissue such as skin, bone, vascular grafts, and cartilage structures. Based upon the desired properties, different materials and formulations can be used to generate both hard and soft tissues. While several 3D printing methods exist, due to the sensitivity of the materials used, extrusion-based methods with bioinks are most commonly employed.
应用
Polaxomer is a thermo-responsive hydrogel which has been used as a mold, track patterning and sacrificial material for bioprinting and tissue engineering. It is considered one of the best printable hydrogels due to the nature of micellar-packing gelation, which allows it to be moved and shifted easily. Moreover, the viscosity of TissueFab®- Sacrificial bioink is stable at both room temperature and human body temperature, which allows it to print at both room temperature and physiological temperature. TissueFab®- Sacrificial Bioink becomes liquid at 4°C and dissolves in aqueous environments, which makes it easy to remove for using as sacrificial material to engineer channels and vasculature in tissue engineering application.
包装
Product contains 10 ml of solution packaged in glass bottle.
其他说明
Important tips for optimal bioprinting results
Procedure
1. Prepare bioink: Keep TissueFab® - Sacrificial Bioink on ice to prevent gelation and gently invert the bioink to make a homogeneous solution. DO NOT vortex or shake vigorously. Transfer TissueFab® - Sacrificial Bioink into the desired printer cartridge.
2. Bioprint: Warm TissueFab® - Sacrificial Bioink in the printer cartridge to room temperature for 10–15 minutes to induce gelation. Follow the 3D printer manufacturer′s instructions. Load the print cartridge onto the 3D printer and print directly onto a Petri dish or into multi-well plates. Adjust the flow according to nozzle diameter, printing speed, printing pressure, and temperature. TissueFab® - Sacrificial Bioink can be printed in tandem with cell laden bioinks using additional printheads.
3. Optional Crosslink: If additional bioinks are used, crosslink the bioprinted structure before removing sacrificial scaffold following bioink instructions.
4. Remove sacrificial scaffold: Cool printed structure to 4 °C for at least 5 minutes. Rinse or perfuse with cold PBS.
5. Culture cells: Culture the bioprinted tissue with appropriate cell culture medium following standard tissue culture procedures.
- Optimize printing conditions (e.g., nozzle diameter, printing speed, printing pressure, temperature, cell density) for the features of your 3D printer and your application.
- Reduce bubble formation. Air bubbles in bioink may hamper bioprinting. Carefully handle the bioink when you mix and transfer it to avoid bubble formation. Do not vortex or shake vigorously.
Procedure
1. Prepare bioink: Keep TissueFab® - Sacrificial Bioink on ice to prevent gelation and gently invert the bioink to make a homogeneous solution. DO NOT vortex or shake vigorously. Transfer TissueFab® - Sacrificial Bioink into the desired printer cartridge.
2. Bioprint: Warm TissueFab® - Sacrificial Bioink in the printer cartridge to room temperature for 10–15 minutes to induce gelation. Follow the 3D printer manufacturer′s instructions. Load the print cartridge onto the 3D printer and print directly onto a Petri dish or into multi-well plates. Adjust the flow according to nozzle diameter, printing speed, printing pressure, and temperature. TissueFab® - Sacrificial Bioink can be printed in tandem with cell laden bioinks using additional printheads.
3. Optional Crosslink: If additional bioinks are used, crosslink the bioprinted structure before removing sacrificial scaffold following bioink instructions.
4. Remove sacrificial scaffold: Cool printed structure to 4 °C for at least 5 minutes. Rinse or perfuse with cold PBS.
5. Culture cells: Culture the bioprinted tissue with appropriate cell culture medium following standard tissue culture procedures.
法律信息
TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 1
法规信息
监管及禁止进口产品
Michael Müller et al.
Journal of visualized experiments : JoVE, (77), e50632-e50632 (2013-07-31)
Bioprinting is an emerging technology that has its origins in the rapid prototyping industry. The different printing processes can be divided into contact bioprinting(1-4) (extrusion, dip pen and soft lithography), contactless bioprinting(5-7) (laser forward transfer, ink-jet deposition) and laser based
Maja Radivojša Matanović et al.
International journal of pharmaceutics, 472(1-2), 262-275 (2014-06-21)
Thermally induced gelling systems have gained enormous attention over the last decade. They consist of hydrophilic homopolymers or block copolymers in water that present a sol at room temperature and form a gel after administration into the body. This article
Bioinks for biofabrication: current state and future perspectives.
Prendergast M E, et al.
Journal of 3D printing in medicine, 1, 49-62 (2016)
Sean V Murphy et al.
Nature biotechnology, 32(8), 773-785 (2014-08-06)
Additive manufacturing, otherwise known as three-dimensional (3D) printing, is driving major innovations in many areas, such as engineering, manufacturing, art, education and medicine. Recent advances have enabled 3D printing of biocompatible materials, cells and supporting components into complex 3D functional
David B Kolesky et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(12), 3179-3184 (2016-03-10)
The advancement of tissue and, ultimately, organ engineering requires the ability to pattern human tissues composed of cells, extracellular matrix, and vasculature with controlled microenvironments that can be sustained over prolonged time periods. To date, bioprinting methods have yielded thin
商品
生物墨水可3D生物打印形成功能组织结构，从而应用于药物筛选、疾病建模和体外移植。针对特定组织工程应用选择生物墨水和打印方法。
Bioinks enable 3D bioprinting of tissue constructs for drug screening and transplantation; select suitable bioinks for specific tissue engineering.
Learn how 3D bioprinting is revolutionizing drug discovery with highly-controllable cell co-culture, printable biomaterials, and its potential to simulate tissues and organs. This review paper also compares 3D bioprinting to other advanced biomimetic techniques such as organoids and organ chips.
Professor Shrike Zhang (Harvard Medical School, USA) discusses advances in 3D-bioprinted tissue models for in vitro drug testing, reviews bioink selections, and provides application examples of 3D bioprinting in tissue model biofabrication.
实验方案
Frequently asked questions (FAQs) for KAPA SYBR® FAST One-Step qRT-PCR Kits.
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