G8168

G418 disulfate salt

Name: G418 disulfate salt
Brand: SIGMA

solution, non-animal origin, suitable for cell culture, BioReagent

别名:

抗生素G418

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关于此项目

经验公式（希尔记法）:

C₂₀H₄₀N₄O₁₀ · 2H₂SO₄

化学文摘社编号:

108321-42-2

分子量:

692.71

MDL number:

MFCD00058314

UNSPSC Code:

12352207

PubChem Substance ID:

24895333

NACRES:

NA.76

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产品名称

G 418 二硫酸盐溶液, 50 mg/mL in H₂O, 0.1 μm filtered, BioReagent, suitable for cell culture

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

biological source

Micromonospora echinospora

sterility

0.1 μm filtered

product line

BioReagent

form

solution

potency

50-60 mg per mL

mol wt

692.7

packaging

pkg of 10 and 100 mL

concentration

50 mg/mL in H₂O

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

colorless

solubility

H₂O: 50 mg/mL, clear

antibiotic activity spectrum

parasites

mode of action

protein synthesis | interferes

storage temp.

2-8°C

Quality Level

200

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Application

G418二硫酸盐是一种氨基糖苷类抗生素，其结构与庆大霉素相似，用于选择和维持原核和真核细胞。不同细胞系具有不同的筛选和维持最佳浓度。细菌和藻类最多需要5 μg/mL而动物细胞则可能需要300-500 μg/mL。

Biochem/physiol Actions

作用机制：G418可通过抑制70S和80S核糖体的合成来阻止多肽合成和蛋白质延伸。

抗菌谱：G418可选择经iNOS启动子构建体和新霉素抗性基因稳定转染的细胞，并显示出对原生动物和蠕虫的活性。

Disclaimer

该产品在2-8°C下可稳定储存两年。

General description

化学结构：氨基糖苷类

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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Journal of cellular and molecular medicine, 23(3), 1784-1797 (2018-12-15)

Aberrant regulation of programmed cell death (PCD) has been tied to an array of human pathologies ranging from cancers to autoimmune disorders to diverse forms of neurodegeneration. Pharmacologic modulation of PCD signalling is therefore of central interest to a number

Direct selection of Pichia pastoris expression strains using new G418 resistance vectors.

Joan Lin-Cereghino et al.

Yeast (Chichester, England), 25(4), 293-299 (2008-03-11)

The methylotrophic yeast, Pichia pastoris, is widely used as a host organism for the expression of heterologous proteins. Currently, the Zeocin and blasticidin resistance genes are the only dominant selectable markers that can be used for primary selection of transformants.

Single-gene imaging links genome topology, promoter-enhancer communication and transcription control.

Jieru Li et al.

Nature structural & molecular biology, 27(11), 1032-1040 (2020-09-23)

Transcription activation by distal enhancers is essential for cell-fate specification and maintenance of cellular identities. How long-range gene regulation is physically achieved, especially within complex regulatory landscapes of non-binary enhancer-promoter configurations, remains elusive. Recent nanoscopy advances have quantitatively linked promoter

Anti-MPER antibodies with heterogeneous neutralization capacity are detectable in most untreated HIV-1 infected individuals.

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Retrovirology, 11, 44-44 (2014-06-10)

The MPER region of the HIV-1 envelope glycoprotein gp41 is targeted by broadly neutralizing antibodies. However, the localization of this epitope in a hydrophobic environment seems to hamper the elicitation of these antibodies in HIV infected individuals.We have quantified and

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Frontiers in immunology, 11, 774-774 (2020-05-21)

We aimed at establishing a sensitive and robust assay for estimation of systemic complement activation at complement component C3 level in mouse and human plasma samples. In order to capture the activation products iC3b and C3dg in a specific and

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G418 disulfate salt

solution, non-animal origin, suitable for cell culture, BioReagent

选择尺寸

关于此项目

主要文件

属性

产品名称

SMILES string

InChI

InChI key

biological source

sterility

product line

form

potency

mol wt

packaging

concentration

technique(s)

impurities

color

solubility

antibiotic activity spectrum

mode of action

storage temp.

Quality Level

相关类别

说明

Application

Biochem/physiol Actions

Disclaimer

General description

安全信息

pictograms

signalword

hcodes

pcodes

Hazard Classifications

存储类别

wgk

flash_point_f

flash_point_c

法规信息

文件

分析证书(COA)

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