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Merck
CN

A9415

放线菌素D

from Streptomyces sp., suitable for cell culture, ≥95%

别名:

放线菌素C1, 放线菌素IV, 更生霉素

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关于此项目

经验公式（希尔记法）:
C62H86N12O16
化学文摘社编号:
50-76-0
分子量:
1255.42
UNSPSC Code:
51282001
NACRES:
NA.76
PubChem Substance ID:
24891447
EC Number:
200-063-6
Beilstein/REAXYS Number:
605235
MDL number:
MFCD00005033

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产品名称

放线菌素D, from Streptomyces sp., suitable for cell culture, ≥95%

InChI key

RJURFGZVJUQBHK-IIXSONLDSA-N

InChI

1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)/t33-,34-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1

SMILES string

CC(C)[C@H]1NC(=O)[C@@H](NC(=O)c2ccc(C)c3OC4=C(C)C(=O)C(N)=C(C(=O)N[C@H]5[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]6CCCN6C(=O)[C@H](NC5=O)C(C)C)C4=Nc23)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]7CCCN7C1=O

biological source

Streptomyces sp.

assay

≥95%

form

powder

technique(s)

cell culture | mammalian: suitable

mp

251-253

solubility

ethanol, DMSO: soluble (Stable in aqueous solutions at 2-8 °C.)

antibiotic activity spectrum

neoplastics

mode of action

DNA synthesis | interferes

storage temp.

2-8°C

Quality Level

300

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相关类别

Application

放线菌素 D 是一种抑制细胞增殖的抗肿瘤抗生素，具有广泛的应用，包括作为细胞培养中的选择剂，用在抑制 HIV 复制和 PC12 细胞程序性死亡的研究中。 细胞培养应用的使用浓度推荐为 1μg/mL。

Biochem/physiol Actions

放线菌素 D 通过与双链 DNA 形成稳定的复合物，抑制 DNA 引发的 RNA 合成并引起 DNA 的单链断裂来抑制细胞的增殖。 研究表明，它是逆转录酶中负链转移步骤的抑制剂。

Disclaimer

该产品具有吸湿性和光敏性。 如果在 2-8°C 下避光保存，经 HPLC 测试，可保持至少 15 个月。 未使用的放线菌素 D 稀溶液对光极为敏感，容易吸附到塑料和玻璃上，应丢弃。 但是，浓缩原液的冷冻等分试样至少可以稳定一个月。

General description

化学结构：肽

Other Notes

保存于密闭容器内，置于干燥通风处。置于干燥处保存。

Preparation Note

放线菌素 D 以红色、有光泽的晶体形式出售，可溶于乙腈或丙酮，浓度为 10 mg/mL，溶于 DMSO 中最低浓度为 1 mg/mL。 一些参考文献还显示出在水中的微弱溶解度为 0.5 mg/mL。

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 2 Oral - Carc. 1B - Repr. 1B

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

法规信息

涉药品监管产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000508208
0000508207
0000508206
0000508207
0000508208

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How do you recommend dissolving Product No. A9415 (Actinomycin D)?

    Stock solutions of this product may be prepared at 10 mg/mL in either ethanol (EtOH) or dimethyl sulfoxide (DMSO).

  6. How long are stock solutions of Actinomycin D (Product No. A9415) stable?

    Stock solutions of this product in DMSO or ethanol should be stored in foil-wrapped vials at -20 °C. In DMSO, solution can be stored for up to 1 month at -20 °C. Do not store in glass vials.

  7. What is the working concentration for Actinomycin D (Product No. A9415)?

    For cell culture applications, actinomycin D is used as a selection agent and is used in banding techniques to differentiate between different regions of chromosomes. Suggested working concentrations is 1 μg/mL.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Antonio Rescifina et al.
Journal of medicinal chemistry, 49(2), 709-715 (2006-01-20)
Isoxazolidinyl polycyclic aromatic hydrocarbons (isoxazolidinyl-PAHs) have been synthesized in good yields by 1,3-dipolar cycloaddition methodology promoted by microwave irradiation. The structures of the obtained cycloadducts have been determined by NOE experiments and supported by computational studies at the AM1 level.
Rolf Schreckenberg et al.
Antioxidants & redox signaling, 23(15), 1220-1232 (2015-05-16)
Research into right ventricular (RV) physiology and identification of pathomechanisms underlying RV failure have been neglected for many years, because function of the RV is often considered less important for overall hemodynamics and maintenance of blood circulation. In view of
Angela Zarama et al.
PLoS pathogens, 10(3), e1004000-e1004000 (2014-03-15)
Receptors of the signalling lymphocyte-activation molecules (SLAM) family are involved in the functional regulation of a variety of immune cells upon engagement through homotypic or heterotypic interactions amongst them. Here we show that murine cytomegalovirus (MCMV) dampens the surface expression
Arkady Khoutorsky et al.
Neuron, 78(2), 298-311 (2013-04-30)
Control of protein synthesis is critical for synaptic plasticity and memory formation. However, the molecular mechanisms linking neuronal activity to activation of mRNA translation are not fully understood. Here, we report that the translational repressor poly(A)-binding protein (PABP)-interacting protein 2A
H Sun et al.
British journal of cancer, 111(2), 281-291 (2014-06-20)
We investigated the biologic and pharmacologic activities of a chromosome region maintenance 1 (CRM1) inhibitor against human non-small cell lung cancer (NSCLC) cells both in vitro and in vivo. The in vitro and in vivo effects of a novel CRM1
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