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Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
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主要文件

安全信息

A9415

Sigma-Aldrich

放线菌素D

from Streptomyces sp., suitable for cell culture, ≥95%

别名:

放线菌素C1, 放线菌素IV, 更生霉素

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100 MG
CN¥6,802.20

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100 MG
CN¥6,802.20

About This Item

经验公式(希尔记法):
C62H86N12O16
CAS号:
分子量:
1255.42
Beilstein:
605235
EC 号:
MDL编号:
UNSPSC代码:
51282001
PubChem化学物质编号:
NACRES:
NA.76

CN¥6,802.20


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获取大包装报价

生物来源

Streptomyces sp.

质量水平

方案

≥95%

表单

powder

技术

cell culture | mammalian: suitable

mp

251-253

溶解性

ethanol, DMSO: soluble (Stable in aqueous solutions at 2-8 °C.)

抗生素抗菌谱

neoplastics

作用机制

DNA synthesis | interferes

储存温度

2-8°C

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此商品
Z561673Z561657Z561681
feature

CE compliant

feature

-

feature

-

feature

CE compliant: no

H × W × D

11 in. × 18 in. × 9 in.

H × W × D

8 in. × 10 in. × 8 in. , 8.5 in. × 10 in. × 8 in.

H × W × D

11 in. × 18 in. × 9 in.

H × W × D

8 in. × 10 in. × 8 in. , 8.5 in. × 10 in. × 8 in.

chamber diam. × L

6 in. × 6 in.

chamber diam. × L

3 in. × 7 in.

chamber diam. × L

6 in. × 6 in. , 6 nL × 6.5 in.

chamber diam. × L

3 in. × 6.5 in. , 3 in. × 7 in.

weight

37 lb

weight

-

weight

37 lb

weight

13 lb

一般描述

化学结构:肽

应用

放线菌素 D 是一种抑制细胞增殖的抗肿瘤抗生素,具有广泛的应用,包括作为细胞培养中的选择剂,用在抑制 HIV 复制和 PC12 细胞程序性死亡的研究中。 细胞培养应用的使用浓度推荐为 1μg/mL。

生化/生理作用

放线菌素 D 通过与双链 DNA 形成稳定的复合物,抑制 DNA 引发的 RNA 合成并引起 DNA 的单链断裂来抑制细胞的增殖。 研究表明,它是逆转录酶中负链转移步骤的抑制剂。

注意

该产品具有吸湿性和光敏性。 如果在 2-8°C 下避光保存,经 HPLC 测试,可保持至少 15 个月。 未使用的放线菌素 D 稀溶液对光极为敏感,容易吸附到塑料和玻璃上,应丢弃。 但是,浓缩原液的冷冻等分试样至少可以稳定一个月。

制备说明

放线菌素 D 以红色、有光泽的晶体形式出售,可溶于乙腈或丙酮,浓度为 10 mg/mL,溶于 DMSO 中最低浓度为 1 mg/mL。 一些参考文献还显示出在水中的微弱溶解度为 0.5 mg/mL。

其他说明

保存于密闭容器内,置于干燥通风处。置于干燥处保存。

象形图

Skull and crossbonesHealth hazard

警示用语:

Danger

危险声明

危险分类

Acute Tox. 2 Oral - Carc. 1B - Repr. 1B

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

法规信息

监管及禁止进口产品

  • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    5. How do you recommend dissolving Product No. A9415 (Actinomycin D)?

      Stock solutions of this product may be prepared at 10 mg/mL in either ethanol (EtOH) or dimethyl sulfoxide (DMSO).

    6. How long are stock solutions of Actinomycin D (Product No. A9415) stable?

      Stock solutions of this product in DMSO or ethanol should be stored in foil-wrapped vials at -20 °C. In DMSO, solution can be stored for up to 1 month at -20 °C. Do not store in glass vials.

    7. What is the working concentration for Actinomycin D (Product No. A9415)?

      For cell culture applications, actinomycin D is used as a selection agent and is used in banding techniques to differentiate between different regions of chromosomes. Suggested working concentrations is 1 μg/mL.

    8. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

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    实验方案

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