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Key Documents

Safety Information

C8302

Sigma-Aldrich

4-Chloro-1-naphthol solution

For HRP detection on Western Blots

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1 G
CN¥1,434.82
5 G
CN¥5,020.05

CN¥1,434.82


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1 G
CN¥1,434.82
5 G
CN¥5,020.05

About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.52

CN¥1,434.82


Please contact Customer Service for Availability

grade

Molecular Biology

Quality Level

form

liquid

storage temp.

2-8°C

SMILES string

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

InChI key

LVSPDZAGCBEQAV-UHFFFAOYSA-N

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OGS624OGS620OGS628
bacteria selection

ampicillin

bacteria selection

ampicillin

bacteria selection

ampicillin

bacteria selection

ampicillin

peptide cleavage

EKT

peptide cleavage

EKT

peptide cleavage

EKT

peptide cleavage

EKT

peptide tag location

C-terminal

peptide tag location

N-terminal

peptide tag location

N-terminal

peptide tag location

C-terminal

origin of replication

pUC

origin of replication

pUC

origin of replication

pUC

origin of replication

pUC

mol wt

size 5897 bp

mol wt

size 5913 bp

mol wt

size 4568 bp

mol wt

size 5923 bp

General description

4-Chloro-1-naphthol is a substrate for HRP in a reaction that results in a colored precipitate. It is commonly used for colorimetric detection and visualization of proteins in western blotting.

Application

Suitable for visualizing protein-peroxidase conjugates in western blotting.

Components

This product contains 0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.

Other Notes

0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.

Principle

Enzymes such as HRP convert 4-Chloro-1-naphthol to a purple-colored precipitate on the blot that is readily visible to the eye.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

163.9 °F - closed cup

Flash Point(C)

73.3 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Hwan-Su Hwang et al.
Planta, 252(3), 44-44 (2020-09-03)
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Animesh Chowdhury et al.
Cell biology international, 44(5), 1142-1155 (2020-01-23)
We sought to determine the mechanism by which angiotensin II (AngII) inhibits isoproterenol induced increase in adenylate cyclase (AC) activity and cyclic adenosine monophosphate (cAMP) production in bovine pulmonary artery smooth muscle cells (BPASMCs). Treatment with AngII stimulates protein kinase

Articles

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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