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Safety Information

D5905

Sigma-Aldrich

3,3′-Diaminobenzidine tetrahydrochloride

peroxidase substrate, chromogenic, tablet

Synonym(s):

3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB

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50 TABS
CN¥3,971.48
100 TABS
CN¥6,794.15
5 X 100 TABS
CN¥25,740.87

CN¥3,971.48

Available to ship onApril 27, 2025Details

Request a Bulk Order
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50 TABS
CN¥3,971.48
100 TABS
CN¥6,794.15
5 X 100 TABS
CN¥25,740.87

About This Item

Linear Formula:
(NH2)2C6H3C6H3(NH2)2 · 4HCl
Molecular Weight:
360.11
Beilstein:
1212988
EC Number:
231-018-9
MDL number:
MFCD00012969
UNSPSC Code:
12352204
PubChem Substance ID:
24893981
NACRES:
NA.83

CN¥3,971.48

Available to ship onApril 27, 2025Details

Request a Bulk Order

Product Name

3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet

Quality Level

200

form

tablet

solubility

water: 1 tablet/15 mL (may contain small insoluble particles)

storage temp.

−20°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

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Show Differences

1 of 4

This Item
T3405D8001D12384
3,3′-Diaminobenzidine tetrahydrochloride tablet, 10 mg substrate per tablet

D5905

3,3′-Diaminobenzidine tetrahydrochloride

3,3′,5,5′-Tetramethylbenzidine dihydrochloride tablet, 1 mg substrate per tablet

T3405

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

3,3′-Diaminobenzidine 97% (HPLC)

D8001

3,3′-Diaminobenzidine

3,3′-Diaminobenzidine 97%

D12384

3,3′-Diaminobenzidine

solubility

water: 1 tablet/15 mL (may contain small insoluble particles)

solubility

-

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

tablet

form

tablet

form

powder

form

powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

-

Specificity

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Application

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.[1][2]
Reagent for spectrophotometric determination of selenium.

Biochem/physiol Actions

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.[3]

Reconstitution

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H302,H319,H341,H350

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品
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    0000410514
    0000389802

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    NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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