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H1009

Sigma-Aldrich

Hydrogen peroxide solution

30 % (w/w) in H2O, contains stabilizer

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About This Item

Empirical Formula (Hill Notation):
H2O2
CAS Number:
7722-84-1
Molecular Weight:
34.01
Beilstein:
3587191
MDL number:
MFCD00011333
UNSPSC Code:
12352304
PubChem Substance ID:
57654227
NACRES:
NA.77

vapor pressure

23.3 mmHg ( 30 °C)

Quality Level

200

Assay

29.0-32.0% (titration)

form

liquid

shelf life

~2 yr

reaction suitability

reagent type: oxidant

concentration

30 % (w/w) in H2O

technique(s)

cell based assay: suitable
immunohistochemistry: suitable

density

1.110 g/cm3

suitability

suitable for DNA precipitation

application(s)

general analytical
pharmaceutical

storage temp.

2-8°C

SMILES string

OO

InChI

1S/H2O2/c1-2/h1-2H

InChI key

MHAJPDPJQMAIIY-UHFFFAOYSA-N

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General description

Hydrogen peroxide (H2O2), a topical antiseptic, is synthesized in aerobic cells as a byproduct of aerobic respiration. It might also be produced as an output of enzymatic reactions in peroxisomes, mitochondria, or other cell compartments.

Application

Hydrogen peroxide solution has been used:
  • to treat nasal epithelial cells for H2O2 sensitivity assays
  • to treat the human immortal keratinocyte cell line (HaCaT) and the human melanoma cell line (GLL19) to induce senescence
  • to incubate brain sections for bromodeoxyuridine (BrdU)/NeuN staining
  • in cell proliferation assay to incubate neural stem cells (NE-4C) for in vitro experiments

Biochem/physiol Actions

Hydrogen peroxide (H2O2) is mainly used in wound cleaning. It can kill the pathogens through oxidation burst and by producing local oxygen. H2O2 functions as a signaling molecule or second messenger. It plays a key role in gene expression in numerous ways. H2O2 participates in modulating inflammation.

Features and Benefits

This compound is featured on the Phosphoprotein Phosphatases (Tyrosine) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
For additional information on our range of Biochemicals, please complete this form.

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Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品
易制爆化学品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
STBL4103
MKCV5253
MKCV9876
SDBB1447
STBL1271

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  1. What is the molarity of the 30% hydrogen peroxide solution?

    The molarity of a 30 % hydrogen peroxide solution is 9.8 M based on a density of 1.11 g/ml.

  2. How stable is the 30 % hydrogen peroxide solution?

    Although dilute solutions of hydrogen peroxide have a limited shelf life, the 30% solution (when properly stored) has a shelf life measured in years. Please see the certificate of analysis for the recommend retest date for a particular lot of Product No. H1009.

  3. Does the hydrogen peroxide contain a stabilizer?

    This product contains 0.5 ppm stannate-containing compounds and 1 ppm phosphorus-containing compounds to stabilize the solution. 

  4. Can hydrogen peroxide be detected spectrophotometrically?

    Hydrogen peroxide has a molar extinction coefficient of 43.6 at 240 nm.

  5. What is the pH of a 30% hydrogen peroxide solution?

    The pH of a 30 % hydrogen peroxide solution is typically between 3 and 4.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

S V Lennon et al.
Cell proliferation, 24(2), 203-214 (1991-03-01)
Cell death may occur by either of two mechanisms: apoptosis or necrosis. Necrosis, the first type of cell death to be recognized, is an uncontrolled degenerative phenomenon invariably caused by noxious stimuli and is the result of irreversible failure of
Akitoshi Nakashima et al.
Scientific reports, 9(1), 11466-11466 (2019-08-09)
Pregnancy is a stress factor culminating into mild endoplasmic reticulum (ER) stress, which is necessary for placental development. However, excessive or chronic ER stress in pre-eclamptic placentas leads to placental dysfunction. The precise mechanisms through which excessive ER stress impacts
Shoichi Iriguchi et al.
Blood, 125(2), 370-382 (2014-10-29)
Although overexpression of T-bet, a master transcription factor in type-1 helper T lymphocytes, has been reported in several hematologic and immune diseases, its role in their pathogenesis is not fully understood. In the present study, we used transgenic model mice
Osamu Sakai et al.
Investigative ophthalmology & visual science, 56(1), 538-543 (2015-01-13)
The purpose of the present study was to investigate the role of glutathione peroxidase 4 (GPx4) in conjunctival epithelial cells. An immortalized human conjunctival epithelial cell line was used. Cells were transfected with catalase, GPx1, GPx4, SOD1, SOD2, or control
Maheswari Jayakannan et al.
Journal of experimental botany, 66(7), 1865-1875 (2015-01-24)
The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which
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