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Key Documents

P7170

Sigma-Aldrich

Ponceau S solution

BioReagent, 0.1 % (w/v) in 5% acetic acid

Synonym(s):

Ponceau S

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1 EA
¥6,780.43

About This Item

Empirical Formula (Hill Notation):
C22H12N4Na4O13S4
CAS Number:
Molecular Weight:
760.57
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

¥6,780.43


Available to ship on2025年4月27日Details


Request a Bulk Order

Product Name

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

description

for protein analysis

Quality Level

product line

BioReagent

form

liquid

concentration

0.1 % (w/v) in 5% acetic acid

technique(s)

western blot: suitable

density

1.01 g/cm3 at 20 °C

suitability

suitable (for use in cellulose acetate electrophoresis)
suitable for electrophoresis

application(s)

diagnostic assay manufacturing
hematology
histology

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1 of 4

This Item
852081852288852347
form

powder

form

powder

form

powder

form

powder

functional group

sulfonic acid

functional group

Fmoc

functional group

Fmoc

functional group

sulfonic acid

product line

Novabiochem®

product line

Novabiochem®

product line

Novabiochem®

product line

Novabiochem®

storage temp.

15-25°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-10 to -25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

reaction suitability

reaction type: Fmoc solid-phase peptide synthesis

General description

Ponceau S is the sodium salt of a diazo dye.[1] Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting).[1] A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Application

Ponceau S solution has been used for membrane staining (Western blotting).[2][3][4]

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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