推荐产品
包装
vial of 6 mL
质量水平
100
200
储存温度
−20°C
相关类别
应用
样品缓冲液，Laemmli 2x 浓缩液已在SDS-PAGE中被用作一种蛋白样品变性和上样的样品缓冲液。
生化/生理作用
Laemmli样品缓冲液是经特殊配方用于Lammli SDS-PAGE系统中蛋白样品的制备。 蛋白样品与2X 样品缓冲液（1:1）混合并在沸水中加热2-5分钟。2-巯基乙醇可还原分子内和分子间的二硫键。 SDS去污剂可使蛋白质和亚基变性，并给每个蛋白质和亚基带来整体的负电荷，以便对它们每个基于大小进行分离。 溴酚蓝可作为一种跑在蛋白前端的染料，也可在上样过程中更加容易的看到样品。 甘油可提高样品的密度以便样本能够铺在样品孔中。
其他说明
含有4% SDS、20% glycerol、10% 2-巯基乙醇、0.004% 溴酚蓝和0.125 M Tris HCl、pH约6.8的溶液。
数量
推荐使用方法：将1倍体积的凝胶上样溶液加入至1倍体积的样品中并充分混匀。
相关产品
产品编号
说明
价格
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
储存分类代码
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Genetic removal of matrix metalloproteinase 9 rescues the symptoms of fragile X syndrome in a mouse model.
Sidhu H, et al.
The Journal of Neuroscience, 34, 9867-9879 (2014)
Amanda M Gonzales et al.
Nutrition & metabolism, 5, 17-17 (2008-06-14)
Adipocytes express inflammatory mediators that contribute to the low-level, chronic inflammation found in obese subjects and have been linked to the onset of cardiovascular disorders and insulin resistance associated with type 2 diabetes mellitus. A reduction in inflammatory gene expression
Dana E Nuti et al.
mBio, 2(4), doi:10-doi:10 (2011-08-19)
Detection of microbial antigens in clinical samples can lead to rapid diagnosis of an infection and administration of appropriate therapeutics. A major barrier in diagnostics development is determining which of the potentially hundreds or thousands of antigens produced by a
GADD45A and EPB41 as tumor suppressor genes in meningioma pathogenesis.
Piaskowski S, et al.
Cancer Genetics and Cytogenetics, 162, 63-67 (2005)
Ibolya Rutkai et al.
Cardiovascular research, 83(1), 148-154 (2009-03-21)
Type 2 diabetes mellitus is frequently associated with hypertension, but the underlying mechanisms are not completely understood. We tested the hypothesis that activation of type 1 prostaglandin E(2) (PGE(2)) receptor (EP1) increases skeletal muscle arteriolar tone and blood pressure in
实验方案
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
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