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Safety Information

S2002

Sigma-Aldrich

Sodium azide

ReagentPlus®, ≥99.5%

Synonym(s):

Hydrazoic acid sodium salt

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About This Item

Linear Formula:
NaN3
CAS Number:
26628-22-8
Molecular Weight:
65.01
EC Number:
247-852-1
MDL number:
MFCD00003536
UNSPSC Code:
12352201
PubChem Substance ID:
24899504
NACRES:
NA.21

biological source

synthetic (inorganic)

Quality Level

200

product line

ReagentPlus®

Assay

≥99.5%

form

powder

storage condition

(Tightly closed. Dry. Keep in a well-ventilated place. Do not store near acids.)

technique(s)

cell based assay: suitable
immunohistochemistry: suitable

color

white

mp

370-425 °C

solubility

soluble 65 g/L at 20 °C (completely)

suitability

suitable for chromatography
suitable for derivatization

application(s)

sample preparation

SMILES string

[Na]N=[N+]=[N-]

InChI

1S/N3.Na/c1-3-2;/q-1;+1

InChI key

PXIPVTKHYLBLMZ-UHFFFAOYSA-N

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General description

Sodium Azide (NaN3) is a potent inhibitor – a water-soluble crystalline powder that′s odorless. Widely recognized for its versatile applications, it plays a pivotal role across molecular biology, cell culture, and biochemical research. Sodium azide is commonly used as a bacteriostatic preservative in aqueous laboratory reagents and biological fluids. It is also a metabolic inhibitor, which inhibits oxidative phosphorylation. In cell culture, it maintains the integrity of your cell lines by preventing microbial contamination. Its application extends to molecular biology, where it aids in preserving nucleic acids for downstream analysis, ensuring the fidelity of your results.

Application

Baylis-Hillman acetates such as ethyl 2-(acetoxyphenylmethyl)acrylate can undergo nucleophilic displacement by sodium azide in aqueous medium to form ethyl (E)-2-azidomethyl-3-phenylpropenoate.
Sodium azide is suitable for use in histopathology to prepare and store tissue samples. Also used as a component of staining buffer (HEPES buffer) for whole mount immunolabelling.
Catalyst for:

  • Oxidative decarboxylation
  • Michael addition reactions

Reagent for synthesis of
  • Blue fluorescent copolymers
  • Metal phosphonates
  • Arenes via aminations

Biochem/physiol Actions

Sodium azide is clinically used as a preservative in diluting fluid for counting red blood cells. It prevents capping and internalization of fluorescent surface-bound antibodies in various scientific applications, including flow cytometry.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

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Signal Word

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - STOT RE 2 Oral

Target Organs

Brain

Supplementary Hazards

EUH032

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

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Certificates of Analysis (COA)

Lot/Batch Number

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  1. What concentrations of Product S2002, Sodium azide, are used as a preservative?

    Typical concentrations for use are from 0.02 % to 0.1 %.

  2. How can I determine the concentration of sodium azide in solution?

    Sodium azide forms a 3,5-dinitrobenzoyl derivative that can be quantified by liquid chromatography.  This method is reported in the Journal of Chromatography, 5, 597 (1982).

  3. Are there any hazards associated with the disposal of Product S2002, Sodium azide?

    Sodium azide can form metal azides.  If you have metal pipes for your plumbing, you should not dispose of solutions down them. 

  4. How stable are solutions of Product S2002, Sodium azide?

    At a neutral pH sodium azide solutions are fairly stable.  Under acidic conditions it is converted to hydrazoic acid, a volatile gas.

  5. How does Product S2002, Sodium azide, inhibit certain metalloenzymes?

    The mechanism of sodium azide inhibition may be due to metal ion complexation and displacement from the enzyme.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Calcium uptake by rat kidney mitochondria.
DeLuca HF and Engstrom GW.
Proceedings of the National Academy of Sciences of the USA, 47(11), 1744-1750 (1961)
Sodium azide, a bacteriostatic preservative contained in commercially available laboratory reagents, influences the responses of human platelets via the cGMP/PKG/VASP pathway.
Russo I, et al.
Clinical Biochemistry, 41(4), 343-349 (2008)
A critical review of published methods for analysis of red cell antigen-antibody reactions by flow cytometry, and approaches for resolving problems with red cell agglutination
Patricia A Arndt , George Garratty
Transfusion Medicine Reviews, 24(3), 174-192 (2010)
Nucleophilic displacement by azide and cyanide on Baylis-Hillman acetates in water.
Yadav JS, et al.
Tetrahedron Letters, 46(16), 2761-2763 (2005)
Abris Gilvesy et al.
Acta neuropathologica, 144(4), 651-676 (2022-08-31)
Tau pathology of the noradrenergic locus coeruleus (LC) is a hallmark of several age-related neurodegenerative disorders, including Alzheimer's disease. However, a comprehensive neuropathological examination of the LC is difficult due to its small size and rod-like shape. To investigate the
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