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Key Documents

Safety Information

S2002

Sigma-Aldrich

Sodium azide

ReagentPlus®, ≥99.5%

Synonym(s):

Hydrazoic acid sodium salt

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1 L
CN¥397.95
2.5 L
CN¥599.59
4 L
CN¥1,336.23
4 L
CN¥1,420.85
10 L
CN¥2,431.86
25 L
CN¥2,845.17

CN¥397.95


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1 L
CN¥397.95
2.5 L
CN¥599.59
4 L
CN¥1,336.23
4 L
CN¥1,420.85
10 L
CN¥2,431.86
25 L
CN¥2,845.17

About This Item

Linear Formula:
NaN3
CAS Number:
Molecular Weight:
65.01
EC Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.21

CN¥397.95


Estimated to ship onApril 22, 2025Details


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biological source

synthetic (inorganic)

Quality Level

product line

ReagentPlus®

Assay

≥99.5%

form

powder

storage condition

(Tightly closed. Dry. Keep in a well-ventilated place. Do not store near acids.)

technique(s)

cell based assay: suitable
immunohistochemistry: suitable

color

white

mp

370-425 °C

solubility

soluble 65 g/L at 20 °C (completely)

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This Item
EZHPIEZHCPEZHGIP
assay range

accuracy: 76-103%, standard curve range: 2-200 μU/mL, linearity: 96-112%

assay range

accuracy: 88-103%, linearity: 84-120%, sensitivity: 0.5 pM, standard curve range: 2-200 pM

assay range

accuracy: 91-111%, linearity: 86-108%, standard curve range: 0.2-20 ng/mL

assay range

accuracy: 86.7%, linearity: 99.9%, sensitivity: 8.2 pg/mL, standard curve range: 8.2-2000 pg/mL

input

sample type plasma (K2 EDTA)
sample type serum
sample type cell culture supernatant

input

sample type serum
sample type plasma (K2 EDTA)

input

sample type plasma (K2 EDTA)
sample type serum

input

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric (450nm)

detection method

colorimetric (450nm)

detection method

colorimetric (450nm)

detection method

colorimetric (450nm/590nm)

General description

Sodium Azide (NaN3) is a potent inhibitor – a water-soluble crystalline powder that′s odorless. Widely recognized for its versatile applications, it plays a pivotal role across molecular biology, cell culture, and biochemical research. It is also a metabolic inhibitor, which inhibits oxidative phosphorylation. In cell culture, it maintains the integrity of your cell lines.

Application

Baylis-Hillman acetates such as ethyl 2-(acetoxyphenylmethyl)acrylate can undergo nucleophilic displacement by sodium azide in aqueous medium to form ethyl (E)-2-azidomethyl-3-phenylpropenoate.[1]
Sodium azide is suitable for use in histopathology to prepare and store tissue samples.[2] [3] Also used as a component of staining buffer (HEPES buffer) for whole mount immunolabelling.[4]
Catalyst for:

  • Oxidative decarboxylation[5]
  • Michael addition reactions[6]

Reagent for synthesis of
  • Blue fluorescent copolymers[7]
  • Metal phosphonates[8]
  • Arenes via aminations[9]

Biochem/physiol Actions

Sodium azide is clinically used as a preservative in diluting fluid for counting red blood cells.[10] It prevents capping and internalization of fluorescent surface-bound antibodies in various scientific applications, including flow cytometry.[11]

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

comparable product

Product No.
Description
Pricing

suggested gloves for splash protection

Signal Word

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - STOT RE 2 Oral

Target Organs

Brain

Supplementary Hazards

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

  • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    1. What concentrations of Product S2002, Sodium azide, are used as a preservative?

      Typical concentrations for use are from 0.02 % to 0.1 %.

    2. How can I determine the concentration of sodium azide in solution?

      Sodium azide forms a 3,5-dinitrobenzoyl derivative that can be quantified by liquid chromatography.  This method is reported in the Journal of Chromatography, 5, 597 (1982).

    3. Are there any hazards associated with the disposal of Product S2002, Sodium azide?

      Sodium azide can form metal azides.  If you have metal pipes for your plumbing, you should not dispose of solutions down them. 

    4. How stable are solutions of Product S2002, Sodium azide?

      At a neutral pH sodium azide solutions are fairly stable.  Under acidic conditions it is converted to hydrazoic acid, a volatile gas.

    5. How does Product S2002, Sodium azide, inhibit certain metalloenzymes?

      The mechanism of sodium azide inhibition may be due to metal ion complexation and displacement from the enzyme.

    6. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    7. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    8. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    9. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    10. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    A critical review of published methods for analysis of red cell antigen-antibody reactions by flow cytometry, and approaches for resolving problems with red cell agglutination
    Patricia A Arndt , George Garratty
    Transfusion Medicine Reviews, 24(3), 174-192 (2010)
    Sodium azide, a bacteriostatic preservative contained in commercially available laboratory reagents, influences the responses of human platelets via the cGMP/PKG/VASP pathway.
    Russo I, et al.
    Clinical Biochemistry, 41(4), 343-349 (2008)
    Nucleophilic displacement by azide and cyanide on Baylis-Hillman acetates in water.
    Yadav JS, et al.
    Tetrahedron Letters, 46(16), 2761-2763 (2005)
    Three-dimensional cellular visualization in mouse apical periodontitis using combined whole-mount staining and optical tissue clearing
    Kento Tazawa , Hajime Sasaki
    Journal of oral biosciences, 65, 132-135 (2023)
    Calcium uptake by rat kidney mitochondria.
    DeLuca HF and Engstrom GW.
    Proceedings of the National Academy of Sciences of the USA, 47(11), 1744-1750 (1961)

    Protocols

    Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.

    Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service