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Key Documents

EZHI

Millipore

Human Insulin ELISA Kit

measures and quantify insulin levels in 20 μL serum or plasma

Synonym(s):

Insulin

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

To inquire about this product EZHI, please contact your local Merck office or dealer. Contact Technical Service

Product Name

Human Insulin ELISA, This Human Insulin ELISA is used to measure & quantify insulin levels in Metabolism & Endocrine research.

Quality Level

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

20 μL sample volume (4hr assay)

assay range

accuracy: 76-103%
linearity: 96-112%
standard curve range: 2-200 μU/mL

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum
sample type cell culture supernatant

NCBI accession no.

UniProt accession no.

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This Item
EZHPIEZHCPEZHGIP
assay range

accuracy: 76-103%, standard curve range: 2-200 μU/mL, linearity: 96-112%

assay range

accuracy: 88-103%, linearity: 84-120%, sensitivity: 0.5 pM, standard curve range: 2-200 pM

assay range

accuracy: 91-111%, linearity: 86-108%, standard curve range: 0.2-20 ng/mL

assay range

accuracy: 86.7%, linearity: 99.9%, sensitivity: 8.2 pg/mL, standard curve range: 8.2-2000 pg/mL

input

sample type plasma (K2 EDTA)
sample type serum
sample type cell culture supernatant

input

sample type serum
sample type plasma (K2 EDTA)

input

sample type plasma (K2 EDTA)
sample type serum

input

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric (450nm)

detection method

colorimetric (450nm)

detection method

colorimetric (450nm)

detection method

colorimetric (450nm/590nm)

General description

Insulin, a 5.8 kDa hormone, is secreted by the islet beta cells in the pancreas.

Application

Human Insulin ELISA has been used to measure the:
  • secretion level of insulin from rat insulinoma (INS-1) cells[1]
  • serum concentrations of insulin[2]
  • insulin secreted from the islet grafts in the mouse serum[3]

Disclaimer

For research use only. Not for use in diagnostic procedures.

Pictograms

CorrosionExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials


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    Stacy R Oliver et al.
    Journal of applied physiology (Bethesda, Md. : 1985), 108(1), 21-27 (2009-10-31)
    In children, exercise modulates systemic anabolism, muscle growth, and overall physiological development through the growth hormone (GH)-insulin-like growth factor I (IGF-I) axis. GH secretion, at rest and during exercise, changes with age and maturational status and can be blunted by
    Maria Luiza Mendonça Pereira Jorge et al.
    Metabolism: clinical and experimental, 60(9), 1244-1252 (2011-03-08)
    The purpose of this study was to compare the effects of 3 different modalities of exercise on metabolic control, insulin resistance, inflammatory markers, adipocytokines, and tissue expression of insulin receptor substrate (IRS)-1 after 12 weeks of training among patients with
    Long-term, progressive, aerobic training increases adiponectin in middle-aged, overweight, untrained males and females.
    Mujumdar PP, Duerksen-Hughes PJ, Firek AF, Hessinger DA
    Scandinavian Journal of Clinical and Laboratory Investigation null
    Total and high molecular weight adiponectin in patients with coronary artery disease.
    Ayman El-Menyar, Nasser Rizk, Abdulrahman D Al Nabti, Sana Abu Hassira, Rajvir Singh et al.
    Journal of Cardiovascular Medicine (Hagerstown, Md.) null
    Michael O Harris-Love et al.
    Journal of clinical medicine, 7(10) (2018-10-13)
    Estimates of muscle tissue composition may have greater prognostic value than lean body mass levels regarding health-related outcomes. Ultrasound provides a relatively low cost, safe, and accessible mode of imaging to assess muscle morphology. The purpose of this study was

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