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N6876

Sigma-Aldrich

Nitrotetrazolium Blue chloride

≥90.0% (HPLC)

Synonym(s):

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

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500 G
CN¥1,370.57
1 KG
CN¥2,127.82

CN¥1,370.57

Available to ship onApril 07, 2025Details

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500 G
CN¥1,370.57
1 KG
CN¥2,127.82

About This Item

Linear Formula:
C40H30N10O6 · 2Cl
CAS Number:
298-83-9
Molecular Weight:
817.64
Beilstein:
4115923
MDL number:
MFCD00012159
UNSPSC Code:
12171500
PubChem Substance ID:
24897795
NACRES:
NA.47

CN¥1,370.57

Available to ship onApril 07, 2025Details

Request a Bulk Order

Quality Level

200

Assay

≥90.0% (HPLC)

form

powder

impurities

≤10% solvent

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

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Show Differences

1 of 4

This Item
XNATD5688XNAT2
Tissue Preparation Solution 30 mL sufficient for 1,000 extractions

T3073

Tissue Preparation Solution

REDExtract-N-Amp™ Tissue PCR Kit sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included

XNAT

REDExtract-N-Amp™ Tissue PCR Kit

Dilution Solution Extract-N-Amp Plant Dilution Solution

D5688

Dilution Solution

Extract-N-Amp™ Tissue PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications

XNAT2

Extract-N-Amp™ Tissue PCR Kit

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

  • Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.[1]
  • It has been used as a substrate for western blot detection.[2]
  • It has been used for the staining of histological tissue sections.[3]

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.[4][5]

Substrates

Substrate for dehydrogenases[6] and other oxidases.[7]

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Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Statements

H302,H315,H319,H335,H371

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2 - STOT SE 3

Target Organs

Eyes,Central nervous system, Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SHBS3161
    MKCN4040
    SHBR6484
    SHBQ0922
    SHBN9436

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Expression of the endothelial lipase gene in murine embryos and reproductive organs.
    Lindegaard ML et al.
    Journal of Lipid Research, 46, 439-439 (2005)
    Measurement of reactive oxygen intermediate production in haemocytes of the penaeid shrimp, Penaeus ?annamei.
    Knaap V et al.
    Aquaculture (Amsterdam, Netherlands), 191 , 89-107 (2000)
    Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump.
    Gaxiola RA et al.
    Proceedings of the National Academy of Sciences of the USA, 98, 11444-11444 (2001)
    Fluorescent in situ hybridization employing the conventional NBT/BCIP chromogenic stain.
    Trinh le A et al.
    Biotechniques, 42, 756-756 (2007)
    Methods in Enzymology, 6, 958-958 (1963)
    View All Related Papers

    Articles

    Colorimetric Phosphatase/Peroxidase Substrate Systems

    NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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