Frequently Asked Questions (FAQs) about Hepatocytes and Other Liver Cells

We offer Stellate cells, Kupffer cells, Liver Sinusoidal Endothelial cells (LSEC), and Intrahepatic Biliary Epithelial cells (Cholangiocytes) to cater to various research needs.

You can find characterization information for our hepatocyte products here, as well as insights into the collection’s key attributes and performance characteristics.

Yes, Safety Data Sheets (SDS) and Certificates of Analysis (COAs) for our hepatocyte products are available. You can access them from the individual product pages.

Yes, all our clinical samples have obtained approval from an Institutional Review Board (IRB). Full documentation is available upon request, including informed consent forms.

Yes, we provide extensive donor information including age, sex, race, diabetic status, smoking history, alcohol use, and any co-morbidities. HLA typing can also be provided for some of the cryo-plateable hepatocytes. Request more information to learn more.

All the liver cells are being tested for HIV, HCV, HBV, and syphilis. Each lot is tested for post-thawing viability and functional assays as indicated by user guide found on the individual product pages.

Yes. However, lot specific reservation orders are not able to be placed over the web. Please contact your local sales rep for more specific details.

Yes, however lot specific orders for donor specific criteria cannot be placed through the web. Please contact your local sales rep for further details.

Please refer to this protocol for instructions on thawing, plating, and culturing plateable hepatocytes. The protocol also details plating and maintenance media formulations. 

Upon receipt, immediately store cryovial or cryovials in vapor phase liquid nitrogen. Cryopreserved human primary hepatocytes are typically viable for at least 5 years when stored in these conditions. Once the vial or vials have been thawed (refer to user guide for protocols for each individual cell type), immediately perform trypan blue count and plate the cells. 

Only our spheroid-qualified hepatocytes (HLP104-4M) should be used for spheroid or other 3D cell culture applications. Not all primary human hepatocytes will work in spheroid and 3D cell culture applications, as there is a donor-to-donor variability in the ability of hepatocytes to form spheroids.

Please refer to this protocol for spheroid-qualified hepatocytes for further information regarding thawing, plating, and culturing spheroid-qualified hepatocytes. The protocol also describes media formulations for culturing spheroid-qualified hepatocytes.

Yes, we offer matched sets of Kupffer cells, Stellate cells, and Cholangiocytes from the same donor as Hepatocytes.  In some cases, we can also offer donor matched PBMCs. Lot specific orders cannot be placed on the web. Please contact your local sales rep for more details.