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HLP302

Sigma-Aldrich

Normal Human Hepatic Stellate Cells; passage 2

Single-Donor Cryopreserved Stellate cells isolated from human livers, high viability, and characterized by cell specific markers

别名:

Homogenous population isolated from whole human livers

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1 KIT
CN¥3,340.17

CN¥3,340.17

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1 KIT
CN¥3,340.17

About This Item

UNSPSC代码:
41106509
NACRES:
NA.81

CN¥3,340.17

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新价格，新优惠！
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生物来源

human liver

表单

frozen liquid

包装

pkg of 1 vial (contains ≥0.25 million cells)
pkg of 1 vial (contains ≥1 million cells)

技术

cell culture | mammalian: suitable

颜色

cloudy

储存温度

-140 to -196°C

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biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

Gene Information

human ... GFAP(2670)

Gene Information

human ... GFAP(2670)

Gene Information

human ... GFAP(2670)

Gene Information

human ... GFAP(2670)

clone

GA5, monoclonal

clone

polyclonal

clone

GA5, monoclonal

clone

GA5, monoclonal

antibody form

purified immunoglobulin

antibody form

serum

antibody form

ascites fluid

antibody form

purified immunoglobulin

isotype

IgG1

isotype

-

isotype

IgG1

isotype

IgG1κ

一般描述

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Hepatic Stellate Cells (HSCs), also called as vitamin A-storing cells, lipocytes, interstitial cells, fat-storing cells, or Ito cells, are liver cells that exist in the space between parenchymal cells and sinusoidal endothelial cells of the hepatic lobule. Stellate cells help store 80% of the vitamin A in the whole body as retinyl palmitate in lipid droplets in the cytoplasm. HSCs are also responsible for vitamin A homeostasis. Primary Hepatic Stellate cells are isolated from whole human livers and are used by researchers to study liver cancer, fibrosis, and liver development and regeneration1. HSCs have a star-like appearance and upon activation can acquire contractile myofibroblast-like morphology, which is an essential step in fibrosis. In fibrosis, activated Stellate cells display a loss of stored vitamin A and increased collagen synthesis, along with other extracellular matrix components.

应用

Stellate cells are ideal in studies of: chronic liver injury, toxicity, activation and differentiation, NAFLD and NASH initiation and progression, fibrosis and more. We offer matched sets of Stellate cells from same donor as Hepatocytes, which can be used for co-culture systems to better simulate the complex microenvironment of the liver and gain insights into various liver-related diseases and conditions.

特点和优势

Our cryopreserved hepatic Stellate cells are derived from the human liver, with each donor providing documented consent for research use of non-transplantable organs or tissues. Cells are cryopreserved at the end of the primary culture. Each lot undergoes testing for specific cell markers and comes with a guarantee of ≥70% post-thaw viability. In addition, all cells have been tested for the absence of HIV, HBV, HCV, Syphillis and microbial contaminants (fungi, bacteria, and mycoplasma).

警告

Each donor is tested and found non-reactive to viral DNA from HIV-1, HIV-2, HTLV I, HTLV II, hepatitis B and hepatitis C. However, no known test can offer complete assurance that the viruses that cause HIV-1, HIV-2, HTLV I, HTLV II, hepatitis B and hepatitis C are not present. Proper precautions and biological containment should be taken when handling cells of human origin, due to their potential biohazardous nature. All human based products should be handled at a BSL-2 (Biosafety Level 2) or higher.

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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    Hepatic stellate cells in liver development, regeneration, and cancer
    Chunyue Yin, Kimberley J Evason, Kinji Asahina, Didier Y R Stainier
    The Journal of Clinical Investigation, 123(5), 1902-1910 (2013)

    商品

    Frequently Asked Questions (FAQs) about Hepatocytes and Other Liver Cells

    Explore answers to frequently asked questions (FAQs) about culturing primary human hepatocytes and other liver cells including Stellate cells, Kupffer cells, Sinusoidal Endothelial cells, and more.

    实验方案

    Primary Human Hepatic Kupffer Cell Culture Protocol

    Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.

    Primary Human Hepatic Sinusoidal Endothelial Cells Culture Protocol

    Discover our collection of primary human hepatic sinusoidal endothelial cells, a protocol for thawing, plating, and growing HHSECs, and a recipe for our HHSEC maintenance media formulation.

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    Primary Human Hepatic Stellate Cells Culture Protocol

    Discover our collection of primary human hepatic stellate cells and protocol for thawing, plating, and growing stellate cells. Find our stellate culture maintenance media formulation.

    Primary Human Intrahepatic Biliary Epithelial Cells (Cholangiocytes) Culture Protocol

    Discover our primary human intrahepatic biliary epithelial cells, also known as cholangiocytes. Explore our plating and maintenance media formulations and protocol for culture.

    我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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