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MAB360

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5

ascites fluid, clone GA5, Chemicon®

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Synonym(s):
GFAP
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GA5, monoclonal

species reactivity

rat, human, mouse, rabbit, chicken, pig, bovine

packaging

antibody small pack of 25 μL

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002055.2

UniProt accession no.

P14136

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GFAP(2670)

Related Categories

General description

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Specificity

Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
Reactivity with other species has not been determined.

Immunogen

Purified GFAP from porcine spinal cord.

Application

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.
Immunocytochemistry:
A previous lot was used on cultured mammalian cells.

Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.

Immunoblotting: A 1:1000 dilution of a previous lot was used.

Optimal working dilutions must be determined by the end user

Quality

Routinely evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.

Target description

~ 51 kDa

Linkage

Replaces: 04-1031; 04-1062

Physical form

Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.

Analysis Note

Control
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

recommended

Product No.
Description
Pricing

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Y Jiang et al.
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Biochip?laser cell deposition system to assess polarized axonal growth from single neurons and neuron?glia pairs in microchannels with novel asymmetrical geometries.
Pirlo, RK; Sweeney, AJ; Ringeisen, BR; Kindy, M; Gao, BZ
Biomicrofluidics null
Jan Gunnar Sørbø et al.
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Protocols

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