Home Small Molecule HPLCHPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL™ LC-18

HPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL™ LC-18

Analysis of Vitamins A and E on SUPELCOSIL™ LC-18

Conditions
column	SUPELCOSIL™ LC-18, 25 cm × 4.6 mm I.D., 5 μm particles (58298)
mobile phase	[A] methanol: [B] deionized water (98:2, A:B)
flow rate	2 mL/min
sample	0.2-1 mg/mL each analyte in methanol
injection	20 μL
detector	UV, 325 nm (retinol, retinyl acetate) or 290 nm (tocopherols, tocopherol acetate)

Description
Categories	Analytical Chromatography, Vitamins and Natural products
Featured Industry	Clinical Vitamins, Nutraceuticals, and Natural Products
Legal Information	SUPELCOSIL™ is a trademark of Sigma-Aldrich Co. LLC
suitability	application for HPLC

Materials

产品编号	产品名称	说明
5.33001	100%乙酸	for LC-MS LiChropur^™
1.00029	乙腈	hypergrade for LC-MS LiChrosolv^®
PHR1031	α-生育酚	Pharmaceutical Secondary Standard; Certified Reference Material
73594	乙酸铵	LiChropur^™, eluent additive for LC-MS
70221	甲酸铵	eluent additive for LC-MS, LiChropur^™, ≥99.0%
1.11727	酒精	gradient grade for liquid chromatography LiChrosolv^®
5.33002	甲酸98％-100％	for LC-MS LiChropur^™
1.06007	甲醇	gradient grade for liquid chromatography LiChrosolv^® Reag. Ph Eur
1.06035	甲醇	hypergrade for LC-MS LiChrosolv^®
5.43841	磷酸二氢钾	for HPLC LiChropur^™
R7632	视黄醇	synthetic, ≥95% (HPLC), (Powder or Powder with Lumps)
95144	视黄醇	BioXtra, ≥97.5% (HPLC), ~3100 U/mg
V-011	维生素A标准液溶液	100 μg/mL ± 25% (Refer to COA) (Ethanol with 0.1% (w/v) BHT), ampule of 1 mL, reference material, Cerilliant^®
R0300000	醋酸维生素 A	European Pharmacopoeia (EP) Reference Standard
46958	视黄醇乙酸酯	analytical standard
PHR1236	视黄醇乙酸酯	Pharmaceutical Secondary Standard; Certified Reference Material
58298	SUPELCOSIL^™ LC-18 HPLC 柱	5 μm particle size, L × I.D. 25 cm × 4.6 mm
47783	(±)-α-生育酚	analytical standard
T1782	(+)-γ-生育酚	≥96% (HPLC)
47785	(+)-γ-生育酚	analytical standard