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column	SUPELCOSIL™ LC-18, 25 cm × 4.6 mm I.D., 5 μm particles (58298)
mobile phase	[A] methanol: [B] deionized water (98:2, A:B)
flow rate	2 mL/min
sample	0.2-1 mg/mL each analyte in methanol
injection	20 μL
detector	UV, 325 nm (retinol, retinyl acetate) or 290 nm (tocopherols, tocopherol acetate)

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Categories	Analytical Chromatography, Vitamins and Natural products
Featured Industry	Clinical \r\nVitamins, Nutraceuticals, and Natural Products
Legal Information	SUPELCOSIL™ is a trademark of Sigma-Aldrich Co. LLC
suitability	application for HPLC

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