HPLC Analysis of Sugars on Ascentis^® Si

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

[A] water; [B] acetonitrile; (15:85, A:B)

1.0 mL/min

35 °C

ELSD, 55 °C, 3.5 bar nitrogen

10 μL

This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany