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HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 15:85)

HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 15:85) application for HPLC

材料

分析色谱柱

产品编号
说明
价格

53981-U

Ascentis® Express HILIC, 2.7 μm HPLC色谱柱

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

标准

产品编号
说明
价格

M8378

麦芽三糖

≥90% (HPLC)

84097

蔗糖

BioUltra, for molecular biology, ≥99.5% (HPLC)

47287-U

乳糖一水合物

analytical standard

G0350500

D -(+)-葡萄糖

European Pharmacopoeia (EP) Reference Standard

X0200000

D-(+)-木糖

European Pharmacopoeia (EP) Reference Standard

1375025

麦芽糖 一水合物

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

说明

分析说明

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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