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HPLC Analysis of Phenolic Compounds on Ascentis® Express 2.7 μm RP-Amide

HPLC Analysis of Phenolic Compounds on Ascentis® Express 2.7 μm RP-Amide application for HPLC

材料

分析色谱柱

产品编号
说明
价格

Ascentis® Express RP-Amide 2.7μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 10 cm × 4.6 mm

标准

产品编号
说明
价格

邻苯二酚

≥99%

邻甲酚

analytical standard

苯酚

PESTANAL®, analytical standard

4-氯-3-甲基苯酚

≥98.0% (HPLC)

Resorcinol

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

对苯二酚

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)

mobile phase

[A] 20 mM phosphoric acid, pH 2; [B] acetonitrile; (75:25, A:B)

flow rate

1.5 mL/min

column temp.

35 °C

detector

UV, 270 nm

injection

25 μL

sample

50 mg/L in mobile phase

说明

分析说明

Chromatography of Seven Phenol Compounds on Ascentis Express RPA

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany