HPLC Analysis of Hydrobenzoin Enantiomers on Astec^® CYCLOBOND^™ I 2000

column

CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)

mobile phase

[A] 20 mM ammonium acetate, pH 4.1; [B] acetonitrile; (80:20, A:B)

flow rate

0.9 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

10 μL

sample

5 mg/mL in methanol