HPLC Analysis of Ancymidol Enantiomers on Astec^® CYCLOBOND^™ I 2000 HP-RSP

column

CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)

mobile phase

[A] 5 mM ammonium acetate, pH 6.0; [B] acetonitrile; (80:20, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 215 nm

injection

5 μL

sample

1 mg/mL in methanol